PDIA1 Antibody

Product Specifications

Background

PDIA1 (protein disulfide isomerase family A member 1) is the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen (1,2) . PDIA1 is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds (3) . Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex (4) .

NCBI Gene ID

5034

Swiss Prot

P07237

Accession Number

NP_000909

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Homeostasis

Purification

PDIA1 antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1468 - Rat Small Intestine Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Rabbit: (100%), Bovine: (100%), Chicken: (88%)

Buffer

PDIA1 antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

PDIA1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

Fragment

IgG

Specificity

PDIA1 antibody is human, mouse and rat reactive.

Symbol

P4HB

NCBI Official Name

Prolyl 4-hydroxylase, beta polypeptid

NCBI Organism

Homo sapiens

Background Reference 01

Pihlajaniemi T, Helaakoski T, Tasanen K, et al. Molecular cloning of the beta-subunit of human prolyl 4-hydroxylase. This subunit and protein disulphide isomerase are products of the same gene. EMBO J. 1987; 6L:643-9.

Background Reference 02

Walmsley AR, Batten MR, Lad U, et al. Intracellular retention of procollagen within the endoplasmic reticulum is mediated by prolyl 4-hydroxylase. J. Biol. Chem. 1999; 274:14884-92.

Background Reference 03

Koivu J, Myllyla R, Halaakoski T, et al. A singel polypeptide acts both as the beta subunit of prolyl 4-hydroxylase and as a protein disulfide-isomerase. J. Biol. Chem. 1987; 262:6447-9.