RFX1 Antibody

Product Specifications

Background

RFX1 is a member of the regulatory factor X protein family, which are transcription factors that contain a highly-conserved winged helix DNA binding domain and the D region found in the C-terminal part of these proteins which facilitates dimerization (1,2) . RFX1 is structurally related to regulatory factors X2, X3, X4, and X5 (3) . RFX1 is a regulatory factor essential for MHC class II genes expression and can bind to an inverted repeat that is required for expression of hepatitis B virus genes (4) . Recent study identifies RFX1 may play a tumor suppressor role in HCC as autophagy mediator (5) .

NCBI Gene ID

5989

Swiss Prot

P22670

Accession Number

NP_002909

Host

Rabbit

Reactivity

Human

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Immunology, Autophagy

Purification

RFX1 antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1201 - HeLa Cell Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Mouse: (89%)

Buffer

RFX1 antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

RFX1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

Observed Molecular Weight

Multiple independent RFX1 antibodies also show a band at ~130 kDa

Fragment

IgG

Specificity

RFX1 antibody is human specific. At least two isoforms of RFX1 are known to exist; this antibody will detect both isoforms.

Symbol

RFX1

Positive Control 2

Cat. No. 17-001 - HeLa Cell Slide

NCBI Official Name

Regulatory factor X, 1 (influences HLA class II expression)

NCBI Organism

Homo sapiens

Background Reference 01

Reith W, Ucla C, Barras E, et al. RFX1, a transactivator of hepatitis B virus enhancer I, belongs to a novel family of homodimeric and heterodimeric DNA-binding proteins. Mol. Cell Biol. 1994; 14:1230-44.

Background Reference 02

Katan-Khaykovich Y and Shaul Y. RFX1, a single DNA-binding protein with a split dimerization domain, generates alternative complexes. J. Biol. Chem. 1998; 273:24504-12.

Background Reference 03

Hsu YC, Liao WC, Kao CY, et al. Regulation of FGF1 gene promoter through transcription factor RFX1. J. Biol. Chem. 2010; 285:13885-95.

Background Reference 04

Su JC, Tseng PH, Hsu CY, et al. RFX1-dependent activation of SHP-1 induces autophagy by a novel obatoclax derivative in hepatocellular carcinoma cells. Oncotarget 2014; 5:4909-19.

Other Product Names

RFX, MHC class II regulatory factor RFX, MHC class II regulatory factor RFX1, enhancer factor C, EFC, trans-acting regulatory factor 1, transcription factor RFX1