DIRAS3 Antibody
Product Specifications
Background
DIRAS3 is a member of the ras superfamily, and is expressed in normal ovarian and breast epithelial cells, but not in ovarian and breast cancers. It is an imprinted gene, with mono-allelic expression of the paternal allele, which is associated with growth suppression and down-regulation of cyclin D1 promoter activity and induction of p21 (WAF/CIP1) . Thus, this gene appears to be a putative tumor suppressor gene whose function is abrogated in ovarian and breast cancers (1) . DIRAS3 has been shown to induce autophagy in human ovarian cancer cells by blocking PI3K signaling, inhibiting the mammalian target of rapamycin (TOR), upregulating ATG4, and colocalizing with LC3 in autophagosomes (2) . DIRAS also interacts with C-RAF and downregulates mitogen-activated protein kinase kinases (MEK) to restrict cell migration (3) .
NCBI Gene ID
9077
Swiss Prot
O95661
Accession Number
NP_004666
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Cell Cycle, Cancer, Autophagy
Purification
DIRAS3 antibody is affinity chromatography purified via peptide column.
Positive Control
Cat. No. 1313 - Human Testis Tissue Lysate
Concentration
1 mg/mL
Buffer
DIRAS3 antibody is supplied in PBS containing 0.02% sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
DIRAS3 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=8139
Fragment
IgG
Specificity
DIRAS3 antibody is human specific.
Symbol
DIRAS3
NCBI Official Name
DIRAS family, GTP-binding RAS-like 3
NCBI Organism
Homo sapiens
Background Reference 01
Yu Y, Xu F, Peng H, et al. NOEY2 (ARHI), an imprinted putative tumor suppressor gene in ovarian and breast carcinomas. Proc. Natl. Acad. Sci. USA 1999; 96:214-9.
Background Reference 02
Lu Z, Luo RZ, Lu Y, et al. The tumor suppressor gene ARHI regulates autophagy and tumor dormancy in human ovarian cancer cells. J. Clin. Invest. 2008; 118:3917-29.
Background Reference 03
Klingauf M, Beck M, Berge U, et al. The tumour suppressor DiRas3 interacts with C-RAF and downregulates MEK activity to restrict cell migration. Biol. Cell 2013; 105:91-107.
Other Product Names
DIRAS family GTP-binding RAS-like 3, ARHI, NOEY2
Tested Applications
ELISA, WB
Protein ID
4757772
Physical Properties
Liquid
Available Sizes
Curated Selection
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