CRBN Antibody

Product Specifications

Background

CRBN, a member of the Lon protease protein family, plays a role in brain development (1) . It is a component of the DCX (DDB1-CUL4-X-box) E3 protein ligase complex, a complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins and is required for limb outgrowth and expression of the fibroblast growth factor FGF8 (2) . CRBN is thought to regulate the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1. It is widely expressed and highly expressed in brain (3,4) .

NCBI Gene ID

51185

Swiss Prot

Q96SW2

Accession Number

NP_057386

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Neuroscience, Stem Cell

Purification

CRBN antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1313 - Human Testis Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Bovine: (94%)

Buffer

CRBN antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

CRBN antibody can be stored at 4˚ C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Specificity

CRBN antibody is human, mouse and rat reactive. At least two isoforms of CRBN are known to exist; this antibody will detect both isoforms.

Symbol

CRBN

NCBI Official Name

Cereblon

NCBI Organism

Homo sapiens

Background Reference 01

Xin W, Xiaohua N, Peilin C, et al. Primary function analysis of human mental retardation related gene CRBN. Mol. Biol. Rep. 2008; 35:251-6.

Background Reference 02

Gandhi AK, Kang J, Havens CG, et al. Immunomodulatory agents lenalidomide and pomalidomide co-stimulate T cells by inducing degradation of T cell repressors Ikaros and Aiolos via modulation of the E3 ubiquitin ligase complex CRL4 (CRBN) . Br. J. Haematol. 2014; 164:811-21.

Background Reference 03

Liu J, Ye J, Zou X, et al. CRL4A (CRBN) E3 ubiquitin ligase restricts BK channel activity and prevents epileptogenesis. Nat. Commun. 2014; 5:3924.

Background Reference 04

Heintel D, Rocci A, Ludwig H, et al. High expression of cereblon (CRBN) is associated with improved clinical response in patients with multiple myeloma treated with lenalidomide and dexamethasone. Br. J. Haematol. 2013; 161:695-700.