ATG4B Antibody

Product Specifications

Background

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2) . ATG4B, also known as AUTL1, is one of four mammalian orthologs of the yeast ATG4 protein; all four are cysteine proteases (3) . ATG4 is required for ATG8 conjugation to phosphatidylethanolamine on autophagosomal membranes. In mammals, each ATG4 homolog shows a selective preference for the ATG8 homologs (4) . ATG4B has been found to be a novel protective protein in inflammatory colitis (5) .

NCBI Gene ID

23192

Swiss Prot

Q9Y4P1

Accession Number

NP_037457

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Autophagy

Purification

ATG4B antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1225 - Ramos Cell Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Bovine: (94%), Chicken: (88%)

Buffer

ATG4B antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

ATG4B antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

Fragment

IgG

Specificity

ATG4B antibody is human, mouse and rat reactive. At least two isoforms of ATG4B are known to exist; this antibody will detect only the larger isoform. ATG4B is predicted to not cross-react with other ATG4 proteins.

Symbol

ATG4B

Positive Control 2

Cat. No. 1205 - Jurkat Cell Lysate

Positive Control 3

Cat. No. 1306 - Human Spleen Tissue Lysate

Positive Control 4

Cat. No. 10-901 - Human Spleen Tissue Slide

NCBI Official Name

Autophagy related 4B, cysteine peptidase

NCBI Organism

Homo sapiens

Background Reference 01

Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene 2004; 23:2891-906.

Background Reference 02

Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.

Background Reference 03

Marino G, Uria JA, Puente XS, et al. Human autophagins, a family of cysteine proteinases potentially implicated in cell degradation by autophagy. J. Biol. Chem. 2003; 278:3671-8.

Background Reference 04

Li M, Hou Y, Wang J, et al. Kinetic comparisons of mammalian Atg4 homologues indicate selective preferences towards diverse Atg8 substrates. J. Biol. Chem. 2011; 286:7327-38.