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RHOT1 Antibody

Product Specifications

Background

The Ras homolog family member T1 (RHOT) is an atypical Rho Ca2+-binding GTPase that localizes to the mitochondria (1) . RHOT1, the related protein RHOT2, the adaptor protein Milton, and the PTEN induced putative kinase 1 (PINK1), form a complex that is involved in axonal transport of mitochondria (2,3) . Both PINK1 and Parkin target RHOT1 for phosphorylation and degradation, causing the arrest of mitochondrial motility (4) .

NCBI Gene ID

55288

Swiss Prot

Q8IXI2

Accession Number

NP_001028740

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Neuroscience

Purification

RHOT1 antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1463 - Rat Brain Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Bovine: (100%), Chicken: (81%)

Buffer

RHOT1 antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

RHOT1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=8027

Fragment

IgG

Specificity

RHOT1 antibody is human, mouse and rat reactive. At least three isoforms of RHOT1 are known to exist; this antibody will detect all three isoforms.

Symbol

RHOT1

NCBI Official Name

Ras homolog family member T1

NCBI Organism

Homo sapiens

Background Reference 01

Fransson A, Ruusala A, and Aspenstrom P. Atypical Rho GTPases have roles in mitochondrial homeostasis and apoptosis. J. Biol. Chem. 2003; 278:6495-502.

Background Reference 02

Macaskill AF, Brickley K, Stephenson FA, et al. GTPase dependent recruitment of Grif-1 by Miro1 regulates mitochondrial trafficking in hippocampal neurons. Mol. Cell. Neurosci. 2009;40:301–12.

Background Reference 03

Weihofen A, Thomas KJ, Ostaszewski B, et al. Pink1 forms a multi-protein complex with Miro and Milton, linking Pink1 function to mitochondrial trafficking. Biochemistry 2009; 48:2045-54.

Background Reference 04

Wang X, Winter D, Ashrafi G, et al. PINK1 and Parkin target Miro for phosphorylation and degradation to arrest mitochondrial motility. Cell 2011; 147:893-906.

Other Product Names

Ras homolog family member T1, mitochondrial Rho GTPase 1, ARHT1, MIRO-1, MIRO1, MIRO

Tested Applications

ELISA, WB, IHC-P, IF

Protein ID

75750480

Physical Properties

Liquid

Available Sizes

Curated Selection

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