IQSEC1 Antibody

Product Specifications

Background

ADP ribosylation factors (Arfs) are small GTP-binding proteins known for their role in vesicular transport, where they nucleate the assembly of coat protein complexes at sites of carrier vesicle formation. IQSEC1 (GEP100/BRAG2), a guanine nucleotide exchange protein for ARF6, belongs to the BRAG family and contains one IQ domain, one PH domain and one SEC7 domain (1) . As a known activator of ARF6, IQSEC1 regulates cell surface levels of Beta1 integrin and its knockdown blocked tumour metastasis (2,3) . IQSEC1 links EGFR signaling to ARF6 activation and may contribute to the cancer metastasis and malignancy (4) .

NCBI Gene ID

9922

Swiss Prot

Q6DN90

Accession Number

NP_055684

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Cell Cycle

Purification

IQSEC1 antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1288 - A20 Cell Lysate

Concentration

1 mg/mL

Buffer

IQSEC1 antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

IQSEC1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

Fragment

IgG

Specificity

IQSEC1 antibody is human, mouse and rat reactive. At least four isoforms of IQSEC1 are known to exist. IQSEC1 antibody is predicted to not cross-react with IQSEC2.

Symbol

IQSEC1

Positive Control 2

Cat. No. 17-208 - A-20 Cell Slide

NCBI Official Name

IQ motif and Sec7 domain 1

NCBI Organism

Homo sapiens

Background Reference 01

Someya A, Sata M, Takeda K, et al. ARF-GEP (100), a guanine nucleotide-exchange protein for ADP-ribosylation factor 6. Proc. Natl. Acad. Sci. U.S.A. 2001; 98:2413-8.

Background Reference 02

Dunphy JL, Moravec R, Ly K, et al. The Arf6 GEF GEP100/BRAG2 regulates cell adhesion by controlling endocytosis of beta1 integrins. Curr. Biol. 2006; 16:315-20.

Background Reference 03

Moravec R, Conger KK, D'Souza R, et al. BRAG2/GEP100/IQSec1 interacts with clathrin and regulates a5B1 integrin endocytosis through activation of ADP ribosylation factor 5 (Arf5) . J. Biol. Chem. 2012; 287:31138-47.

Background Reference 04

Hu Z, Du J, Yang L, et al. GEP100/Arf6 is required for epidermal growth factor-induced ERK/Rac1 signaling and cell migration in human hepatoma HepG2 cells. PLoS One 2012; 7:e38777.