SAMHD1 Antibody

Product Specifications

Background

The SAM domain and HD domain 1 (SAMHD1) protein is upregulated in response to viral infection and is thought to play a role in innate immunity (1) . SAMHD1 blocks the infection of HIV-1 and SIVdeltaVpx before reverse transcription in macrophages and dendritic cells (2), and this restriction is regulated by phosphorylation of SAMHD1 (3) . Mutations in this gene have been associated with Aicardi-Goutieres syndrome (1) .

NCBI Gene ID

25939

Swiss Prot

Q9Y3Z3

Accession Number

NP_056289

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

8007

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Validation

KO Validation (Figure 5) shows SAMHD1 expression detected by anti-SAMHD1 antibodies (8007) was disrupted in multiple cells of SAMHD1 KO mice (figure 6) . KO Validation (8007) Overexpression validation (Figure 2,6,7) : SAMHD1 overexpression detected by anit-SAMHD1 antibodies (8007) was observed in normal cell lines stably across all healthy donors. Overexpression validation8007 Regulated expression validation (Figure 8 & 9) : SAMHD1 expression detected by anit-SAMHD1 antibodies (8007) was down-regulated by nucleofection with miR-181b at 48hr (figure 8), but up-regulated by treatment of miR-181family inhibitor (figure 9) . Regulated expression validation8007

Field of Research

Innate Immunity

Purification

SAMHD1 antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1224 - Daudi Cell Lysate

Concentration

1 mg/mL

Buffer

SAMHD1 antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

SAMHD1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

Fragment

IgG

Specificity

SAMHD1 antibody is human and mouse reactive.

Isoform

Human SAMHD1 has 3 isoforms, including isoform 1 (626aa, 72kD), isoform 3 (556aa, 64kD), isoform 4 (591aa, 68kD) . Mouse SAMHD1 has 2 isoforms, including (658aa, 76kD) and (651aa, 75kD) . Rat SAMHD1 has only one isoform (620aa, 72kD) . 8007 can detect human, mouse and rat.

Symbol

SAMHD1

NCBI Official Name

SAM domain and HD domain 1

NCBI Organism

Homo sapiens

Background Reference 01

Rice et al. Nat. Genet. 2009; 41:829-32.

Background Reference 02

Hrecka et al. Nature 2011; 474:654-7.

Background Reference 03

Welbourn et al. J. Virol. 2013; 87:11516-24.

Citation 01

29911928

Citation 02

27889686

Citation 03

29321329

Citation 04

26580513

Other Product Names

SAM domain and HD domain 1, DCIP, CHBL2, HDDC1, MOP-5, SBBI88

Tested Applications

ELISA, WB, IHC, IF

Protein ID

38016914

Physical Properties

Liquid

Available Sizes

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