SAMHD1 (phospho Thr592) Antibody

Product Specifications

Background

The SAM domain and HD domain 1 (SAMHD1) protein is upregulated in response to viral infection and is thought to play a role in innate immunity (1) . SAMHD1 blocks the infection of HIV-1 and SIVdeltaVpx before reverse transcription in macrophages and dendritic cells (2), and this restriction is regulated by phosphorylation of SAMHD1 (3) . Mutations in this gene have been associated with Aicardi-Goutieres syndrome (1) .

NCBI Gene ID

25939

Swiss Prot

Q9Y3Z3

Accession Number

NP_056289

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

8005

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Validation

Mutant validation (Figure 2, 5, 6 & 7) : Anti-SAMHD1 (phospho Thr592) antibody (8005) specificity was further verified by SAMHD1 (phospho Thr592) mutant. SAMHD1 (phospho Thr592) signal in 293T cells (figure 2) or HEK293T cells (figure 5 & 7) or U937 cells (figure 6) transfected with SAMHD1 (phospho Thr592) mutant was disrupted in comparison with that in cells transfected with the wild-type. Mutant validation8005 KD validation (Figure 8) : SAMHD1 (phospho Thr592) expression detected by anit-SAMHD1 (phospho Thr592) antibodies (8005) was disrupted in knockdown macrophages. KD validation8005 Regulated expression validation (Figure 10) : SAMHD1 (phospho Thr592) expression detected by anit-SAMHD1 (phospho Thr592) antibodies (8005) was down-regulated by lambda-phosphatase treatment in S and G2 phase of cell cycle. Regulated expression validation8005

Field of Research

Immunology, Innate Immunity

Purification

SAMHD1 (phospho Thr592) antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 10-301 - Human Brain Tissue Slide

Concentration

1 mg/mL

Buffer

SAMHD1 (phospho Thr592) antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

SAMHD1 (phospho Thr592) antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

Fragment

IgG

Specificity

Phospho-SAMHD1 antibody will only detect the phosphorylated form of SAMHD1.

Isoform

Human SAMHD1 (phospho Thr592) has 3 isoforms, including isoform 1 (626aa, 72kD), isoform 3 (556aa, 64kD), isoform 4 (591aa, 68kD) . Mouse SAMHD1 has 2 isoforms, including (658aa, 76kD) and (651aa, 75kD) . Rat SAMHD1 has only one isoform (620aa, 72kD) . 8005 can detect human and mouse.

Symbol

SAMHD1 (phospho Thr592)

NCBI Official Name

SAM domain and HD domain 1

NCBI Organism

Homo sapiens

Background Reference 01

Rice et al. Nat. Genet. 2009; 41:829-32.

Background Reference 02

Hrecka et al. Nature 2011; 474:654-7.

Background Reference 03

Welbourn et al. J. Virol. 2013; 87:11516-24.

Citation 01

27711056

Citation 02

30132758

Citation 03

29321329

Citation 04

30039733

Citation 05

28122869

Citation 06

29084722

Citation 07

29610582

Citation 08

30068654

Citation 09

27815502

Citation 10

26667483

Citation 11

Feifei Wang. Comparison of Murine and Human SAMHD1's Role in Retroviral Restriction and Cell Cycle Progression. PhD Thesis. The Ohio State University. 2015.

Citation 12

Alexandra Herrmann. The role of SAMHD1 in restriction and immune sensing of retroviruses and retroelements. PhD Thesis. The Friedrich Alexander University Erlangen-Nuremberg. 2018.