Nephrin Antibody

Product Specifications

Background

Nephrin is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth (1) . Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm (2,3) .

NCBI Gene ID

4648

Swiss Prot

O60500

Accession Number

NP_004637

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

7979

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Validation

Induced Expression Validation (Figure 6) : Nephrin expression detected by anit-Nephrin antibodies was up-regulated by curcumin treatment in type 1 diabetic rats. Induced Expression Validation Localization Validation (Figure 7) : Nephrin detected by anti-Nephrin antibodies is colocalized with PKGIα at the tips of the cell processes in rat podocytes. Localization Validation Regulated expression validation (Figure 10) : Nephrin expression detected by anit-Nephrin antibodies was down-regulated by PEGF treatment (NGP or HGP), which was reversed by the addition of C3 transferase in mouse podocyte cells. Regulated expression validation

Field of Research

Homeostasis

Purification

Nephrin antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1303 - Human Brain Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Rat (93%) .

Buffer

Nephrin antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Nephrin antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

Fragment

IgG

Isoform

Human Nephrin has 2 isoforms, including isoform 1 (1241aa, 135kD) and isoform 2 (1201aa, 131kD) . Mouse Nephrin also has 2 isoforms, including isoform 1 (1256aa, 136kD) and isoform 2 (1242aa, 135kD) . Rat Nephrin has 3 isoforms, including isoform 1 (1252aa, 136kD), isoform 2 (1239aa, 135kD) and isoform 3 (1166aa, 127kD) . 7979 can detect human and mouse.

Symbol

NPHS1

Positive Control 2

Cat. No. 1201 - HeLa Cell Lysate

Positive Control 3

Cat. No. 1224 - Daudi Cell Lysate

Positive Control 4

Cat. No. 1285 - C2C12 Cell Lysate

NCBI Official Name

Nephrosis 1, congenital, Finnish type (nephrin)

NCBI Organism

Homo sapiens

Background Reference 01

Kestila et al. Mol. Cell 1998; 1:575-582.

Background Reference 02

Tryggvason. J. Am. Soc. Nephrol. 1999; 10:2440-5.

Background Reference 03

Tryggvason and Wartiovaara. Curr. Opin. Nephrol. Hypertens. 2001; 10:543-9.

Citation 01

24004644

Citation 02

22898567

Citation 03

23454089

Citation 04

22212473

Citation 05

30536192

Citation 06

21520075

Citation 07

29032153

Other Product Names

NPHN, NPHS1, Renal glomerulus-specific cell adhesion receptor3

Tested Applications

ELISA, WB, IHC-P, IF

Protein ID

4758822

Physical Properties

Liquid

Available Sizes

Curated Selection

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