AKAP4 Antibody

Product Specifications

Background

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell (1) . AKAP4, also known as hAKAP82, is a major sperm fibrous sheath protein that localizes to the entire length of the flagellum of human sperm (2) . It has been suggested that AKAP4 plays multiple roles in sperm motility and regulation of signal transduction pathways (2) . AKAP4 has also been postulated to be a potential biomarker for breast cancer (3) .

NCBI Gene ID

8852

Swiss Prot

Q5JQC9

Accession Number

NP_003877

Host

Rabbit

Reactivity

Human

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Signal Transduction

Purification

AKAP4 antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1313 - Human Testis Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Rat: (94%), Mouse: (88%)

Buffer

AKAP4 antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

AKAP4 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

Fragment

IgG

Specificity

AKAP4 antibody is human specific. At least three isoforms are known to exist; this antibody will detect the two longest isoforms.

Symbol

AKAP4

Positive Control 2

Cat. No. 11-701 - Human Testis Tissue Slide

NCBI Official Name

A kinase (PRKA) anchor protein 4

NCBI Organism

Homo sapiens

Background Reference 01

Carnegie GK, Means CK, and Scott JD. A-kinase anchoring proteins: from protein complexes to physiology and disease. IUBMB 2009; 61:394-406.

Background Reference 02

Turner RM, Johnson LR, Haig-Ladewig L, et al. An X-linked gene encodes a major human sperm fibrous sheath protein, hAKAP82. Genomic organization, protein kinase A-RII binding, and distribution of the precursor in the sperm tail. J. Biol. Chem. 1998; 273:32135-41.

Background Reference 03

Saini S, Jagadish N, Gupta A, et al. A novel cancer testis antigen, A-kinase anchor protein 4 (AKAP4) is a potential biomarker for breast cancer. PLoS One 2013; 8:e57095.