TRIP12 Antibody

Product Specifications

Background

Thyroid hormone receptors (TRs) are transcription factors that regulate the expression of specific genes in a hormone-dependent manner (1) . TRIP12 (thyroid hormone receptor interactor 12) is an ATP-dependent E3 ubiquitin ligase involved in the human ubiquitin fusion degradation (UFD) pathway and also modulates the NEDD8 pathway (2,3) . TRIP12 contains one WWE domain and a single HECT (E6AP-type E3 ubiquitin-protein ligase) domain suggested to contain a noncovalent ubiquitin-binding site (4) . TRIP12 acts as a key regulator of DNA damage response and the ubiquitin ligase activity of TRIP12 is essential for mouse development (5) .

NCBI Gene ID

9320

Swiss Prot

Q14669

Accession Number

NP_004229

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Homeostasis, Stem Cell

Purification

TRIP12 antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1472 - Rat Colon Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Bovine: (100%)

Buffer

TRIP12 antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

TRIP12 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

Fragment

IgG

Specificity

TRIP12 antibody is human, mouse and rat reactive. At least four isoforms are known to exist.

Symbol

TRIP12

NCBI Official Name

Thyroid hormone receptor interactor 12

NCBI Organism

Homo sapiens

Background Reference 01

Lee JW, Choi HS, Gyuris J, et al. Two classes of proteins dependent on either the presence or absence of thyroid hormone for interaction with the thyroid hormone receptor. Mol. Endocrinol. 1995; 9:243–54.

Background Reference 02

An CI, Ganio E, and Hagiwara N. Trip12, a HECT domain E3 ubiquitin ligase, targets Sox6 for proteasomal degradation and affects fiber type-specific gene expression in muscle cells. Skelet. Muscle 2013; 3:11.

Background Reference 03

Poulsen EG, Steinhauer C, Lees M, et al. HUWE1 and TRIP12 collaborate in degradation of ubiquitin-fusion proteins and misframed ubiquitin. PLoS One. 2012; 7:e50548.

Background Reference 04

Park Y, Yoon SK, and Yoon JB. The HECT domain of TRIP12 ubiquitinates substrates of the ubiquitin fusion degradation pathway. J. Biol. Chem. 2009; 284:1540-9.