SMURF1 Antibody

Product Specifications

Background

SMURF1 (SMAD ubiquitin regulatory factor 1) is a negative regulator of TGF-beta signaling (1) . SMURF1 and SMURF2 are members of HECT domain E3 ubiquitin ligase which are involved in the enzymatic reactions of the Ub conjugating pathway (1,2) . SMURF1 negatively regulates osteoblast activity and response to bone morphogenesis protein (BMP) through controlling MEKK2 degradation (3) . SMURF1 has recently been shown to play a role in breast cancer cell migration and invasion through the downregulation of RhoA (4) .

NCBI Gene ID

57154

Swiss Prot

Q9HCE7

Accession Number

NP_065162

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Signal Transduction, Cancer

Purification

SMURF1 antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1210 - HEK293 Cell Lysate

Concentration

1 mg/mL

Buffer

SMURF1 antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

SMURF1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

Fragment

IgG

Specificity

SMURF1 antibody is human, mouse and rat reactive. At least three isoforms of SMURF1 are known to exist this antibody will recognize all three isoforms. SMURF1 is predicted to not cross-react with SMURF2.

Symbol

SMURF1

NCBI Official Name

SMAD specific E3 ubiquitin protein ligase 1

NCBI Organism

Homo sapiens

Background Reference 01

Huibregtse JM, Scheffner M, Beaudenon S, et al. A family of proteins structurally and functionally related to the E6-AP ubiquitin-protein ligase. Proc. Natl. Acad. Sci. USA 1995; 92:2563-7.

Background Reference 02

Hwang YS, Lee HS, Kamata T, et al. The Smurf ubiquitin ligases regulate tissue separation via antagonistic interactions with ephrinB1. Genes Dev. 2013; 27:491-503.

Background Reference 03

Yamashita M, Ying SX, Zhang G, et al. Ubiquitin ligase Smurf1 controls osteoblast activity and bone homeostasis by targeting MEKK2 for degradation. Cell 2005; 121:101-113.