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Vinculin Antibody

Product Specifications

Background

Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development (1) . Three structural vinculin domains include an amino-terminal head, a short flexible proline-rich region and a carboxy-terminal tail (2) . Expression of vinculin were shown to be affected by the level of actin expression (2,3) . Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth (4) . Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) (5) .

NCBI Gene ID

7414

Accession Number

NP_003364

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

7807

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Validation

Independent Antibody Validation in Cell lines (Figure 1) shows similar Vinculin expression profile in human, mouse and rat cell lines detected by three independent anti-Vinculin antibodies that recognize different epitopes, 7489 against C-terminus domain, 7807 against N-terminus domain and 7809 against central domain. Vinculin proteins are detected in the most tested cell lines at different expression levels by the three independent antibodies. Independent Antibody Validation in Cell lines7489 78077809

Field of Research

Homeostasis

Purification

Vinculin antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1202 - A431 Cell Lysate

Concentration

1 mg/mL

Buffer

Vinculin antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Vinculin antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=7807

Fragment

IgG

Specificity

Vinculin antibody is human, mouse and rat reactive. At least three isoforms of Vinculin are known to exist.

Isoform

Human Vinculin has 3 isoforms, including isoform 2 (1134aa, 124kD), isoform 1 (1066aa, 117kD) and isoform 3 (222aa, 25kD) . Mouse Vinculin has one isoform (1066aa, 117kD) and Rat Vinculin also has one isoform (1066aa, 117kD) . 7807 can detect human, mouse and rat.

Symbol

VCL

Positive Control 2

Cat. No. 1201 - HeLa Cell Lysate

Positive Control 3

Cat. No. 1282 - 3T3/NIH Cell Lysate

NCBI Official Name

Vinculin

NCBI Organism

Homo sapiens

Background Reference 01

Burridge et al. Annu. Rev. Cell Biol.1988; 4:487-525.

Background Reference 02

Gilmore et al. J. Cell Sci. 1992; 103:719-31.

Background Reference 03

Deakin et al. PLoS One 2012; 7:e37990.

Background Reference 04

Goldmann et al. Cell Biol. Int. 2013; Feb 1.

Citation 01

26873631

Other Product Names

CMD1W, CMH15, Metavinculin, MVCL

Tested Applications

ELISA, WB, ICC, IF

Protein ID

4507877

Physical Properties

Liquid

Available Sizes

Curated Selection

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