DEPTOR Antibody

Product Specifications

Background

DEPTOR is an mTOR (mammalian target of rapamycin) -interacting protein whose expression is negatively regualted by mTOR complex 1 (mTORC1) and mTORC2 (1) . Loss of DEPTOR activates RP6SK1, Akt1, and SGK1, promotes cell growth and survival, and activates mTORC1 and mTORC2 kinase activities. DEPTOR expression is low in most cancers, but is often elevated in multiple myelomas (1) . In respons to stimulation by mitogens, DEPTOR is rapidly phosphorylated leading to its ubiquitination and degradation, allowing mTOR to generate an auto-amplification loop to promotes its own full activation (2) .

NCBI Gene ID

64798

Swiss Prot

Q8TB45

Accession Number

NP_073620

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Homeostasis

Purification

DEPTOR antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1404 - Mouse Liver Tissue Lysate

Concentration

1 mg/mL

Buffer

DEPTOR Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

DEPTOR antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

Fragment

IgG

Specificity

DEPTOR antibody is human, mouse and rat reactive. At least three isoforms of DEPTOR are known to exist; this antibody will detect all three isoforms.

Symbol

DEPTOR

NCBI Official Name

DEP domain containing MTOR-interacting protein

NCBI Organism

Homo sapiens

Background Reference 01

Peterson TR, Laplante M, Thoreen CC, et al. DEPTOR is an mTOR inhibitor frequently overexpressed in multiple myeloma cells and required for survival. Cell 2009; 137:873-6.

Background Reference 02

Duan S, Skaar JR, Kuchay S, et al. mTOR generates an auto-amplification loop by triggering the bTrCP- and CK1a-dependent degradation of DEPTOR. Mol. Cell 2011; 44:317-24.