TRIM21 Antibody

Product Specifications

Background

TRIM21, a member of the tripartite motif (TRIM) family, is a part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus (1) . RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus (2) . TRIM21 also acts as a cytosolic antibody receptor; detection of intracellular antibodies activate immune signaling by stimulating the transcription factor pathways of NF-kappaB, AP-1, IRF3, IRF5, and IRF7, resulting in the production of proinflammatory cytokines and the induction of an antiviral state (3) .

NCBI Gene ID

6737

Swiss Prot

P19474

Accession Number

NP_003132

Host

Rabbit

Reactivity

Human

Immunogen

7729

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Validation

Independent Antibody Validation in Cell lines (Figure 2) shows similar TRIM21 expression profile in human cell lines detected by two independent anti-TRIM21 antibodies that recognize different epitopes, 7729 against central domain and 18-413 against recombinant fusion protein. TRIM21 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines7729 18-413

Field of Research

Innate Immunity

Purification

TRIM21 antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1302 - Human Lung Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Bovine (73%), Mouse (69%) and Rat (69%) .

Buffer

TRIM21 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

TRIM21 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

Fragment

IgG

Specificity

TRIM21 antibody is human specific. This antibody is predicted to not cross-react with TRIM6.

Isoform

Human TRIM21 has 2 isoforms, including isoform 1 (475aa, 54kD) and isoform 2 (398aa, 45kD) . Mouse TRIM21 has one isoform (470aa, 54kD) and Rat TRIM21 also has one isoform (471aa, 54kD) . 7729 can detect human and possibly mouse and rat.

Symbol

TRIM21

Positive Control 2

Cat. No. 11-801 - Human Small Intestine Tissue Slide

Positive Control 3

Cat. No. 1201 - HeLa Cell Lysate

Positive Control 4

Cat. No. 1220 - SK-N-SH Cell Lysate

NCBI Official Name

Tripartite motif containing 21

NCBI Organism

Homo sapiens

Background Reference 01

Oke and Wahren-Herlenius. J. Autoimmun. 2012; 39:77-82.

Background Reference 02

Frank et al. Biochem. J. 1995; 305:359-62.

Background Reference 03

McEwan. Nat. Immunol. 14:327-36.

Other Product Names

TRIM21 Antibody: SSA, RO52, SSA1, RNF81, Ro/SSA, E3 ubiquitin-protein ligase TRIM21, 52 kDa Ro protein, SS-A

Tested Applications

ELISA, WB, IHC-P, IF

Protein ID

15208660

Physical Properties

Liquid

Available Sizes

Curated Selection

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