ME1 Antibody

Product Specifications

Background

ME1, also known as NADP-ME, MES or HUMNDME, may play an important role as a housekeeping protein within the cell (1) . ME1 is a 572 amino acid cytoplasmic protein that belongs to the malic enzyme family (2) . It is expressed ubiquitously with highest expression in liver and white adipose tissue. ME1 functions as an NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis (3) . The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles (3,4) . ME1 is regulated by both thyroid hormone levels and the amount of carbohydrates in the diet (5) .

NCBI Gene ID

4199

Swiss Prot

P48163

Accession Number

NP_002386

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Homeostasis

Purification

ME1 antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1210 - HEK293 Cell Lysate

Concentration

1 mg/mL

Buffer

ME1 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

ME1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

Fragment

IgG

Specificity

ME1 antibody is human, mouse and rat reactive. ME1 antibody is predicted not to cross-react with ME2.

Symbol

ME1

NCBI Official Name

Malic enzyme 1, NADP (+) -dependent, cytosolic

NCBI Organism

Homo sapiens

Background Reference 01

Loeber G, Dworkin MB, Infante A, et al. Characterization of cytosolic malic enzyme in human tumor cells. FEBS Lett. 1994; 344:181-6.

Background Reference 02

Gonzalez-Manchon C, Ferrer M, Ayuso MS, et al. Cloning, sequencing and functional expression of a cDNA encoding a NADP-dependent malic enzyme from human liver. Gene 1995;159:255-60.

Background Reference 03

Gonzalez-Manchon C, Butta N, Ferrer M, et al. Molecular cloning and functional characterization of the human cytosolic malic enzyme promoter: thyroid hormone responsiveness. DNA Cell Biol. 1997;16:533-44.

Background Reference 04

Yang Z, Lanks CW and Tong L. Molecular mechanism for the regulation of human mitochondrial NAD (P) +-dependent malic enzyme by ATP and fumarate. Structure 2002; 10:951-60.