HMGB1 Antibody

Product Specifications

Background

The high-mobility group box 1 (HMGB1) protein was originally identified as a highly conserved nuclear DNA-binding protein that participates in DNA replication, repair and transcriptional regulation of gene expression (1,2) . However, HMGB1 has more recently emerged as an extracellularly released mediator of inflammation and repair responses in lipopolysaccharide (LPS) -induced endotoxemia and sepsis (3) . HMGB1 has also been suggested to induce secretion of pro-inflammatory cytokines and chemokines by inducing NF-kB signaling downstream of Toll-like receptor (TLR) 2 and TLR4 activation (4) .

NCBI Gene ID

3146

Swiss Prot

P09429

Accession Number

NP_002119

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

7715

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Validation

KO Validation (Figure 1) : Anti-HMGB1 antibodies (7715) specificity was further verified by HMGB1 specific knockout cell lysate. HMGB1 signal was detected in wild type cells, but not in the HMGB1 knockout cells. KO Validation7715)

Field of Research

Innate Immunity

Purification

HMGB1 antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1303 - Human Brain Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Chicken: (100%), Pig: (100%)

Buffer

HMGB1 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

HMGB1 antibody can be stored at 4˚ C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Specificity

HMGB1 antibody is human, mouse and rat reactive.

Isoform

Human HMGB1 has only 1 isoform (215aa, 25kD) . Mouse HMGB1 has only 1 isoform (215aa, 25kD) . Rat HMGB1 has only 1 isoform (215aa, 25kD) . This antibody detects HMGB1 of human, mouse and rat.

Symbol

HMGB1

Positive Control 2

Cat. No. 10-101 - Human Lung Tissue Slide

NCBI Official Name

High mobility group box 1

NCBI Organism

Homo sapiens

Background Reference 01

Smerdon MJ and Isenberg I. Interactions between the subfractions of calf thymus H1 and nonhistone chromosomal proteins HMG1 and HMG2. Biochemistry 1976; 15:4242-7.