AQP2 Antibody

Product Specifications

Background

Aquaporins are membrane proteins that serve in the transfer of water and small solutes across cellular membranes. One such aquaporin, aquaporin-2 (AQP2) is located in the kidney collecting tubule and plays a critical role in water reabsorbtion (1) . AQP2 is mainly localized in intracellular vesicles but upon stimulation with anti-diuretic hormone (ADH), AQP2 is translocated to the apical plasma membrane by exocytic fusion of AQP2-bearing vesicles (2) . Mutations in this gene have been linked to autosomal dominant and recessive forms of nephrogenic diabetes insipidus (3) .

NCBI Gene ID

359

Swiss Prot

P41181

Accession Number

NP_000477

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Homeostasis

Purification

AQP2 antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1202 - A431 Cell Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Sheep: (100%), Bovine: (95%)

Buffer

AQP2 antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

AQP2 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

Fragment

IgG

Specificity

AQP2 antibody is human, mouse and rat reactive. This antibody is predicted to not cross-react with other members of the aquaporin protein family.

Symbol

AQP2

Positive Control 2

Cat. No. 10-401 - Human Kidney Tissue Slide

NCBI Official Name

Aquaporin 2 (collecting duct)

NCBI Organism

Homo sapiens

Background Reference 01

Denker BM, Smith BL, Kuhada FP, et al. Identification, purification, and partial characterization of a novel Mr 28,000 integral membrane protein from erythrocytes and renal tubules. J. Biol. Chem. 1988; 263:15634-42.

Background Reference 02

Barile M, Pisitkun T, Yu MJ, et al. Large scale protein identification in intracellular aquaporin-2 vesicles from renal inner medullary collecting duct. Mol. Cell Proteomics 2005; 4:1095-106.

Background Reference 03

Bockenhauer D and Bichet DG. Inherited secondary nephrogenic diabetes insipidus: concentrating on humans. Am. J. Physiol. Renal Physiol. 2013; 304:F1037-42.