PHC3 Antibody

Product Specifications

Background

Polycomb group (PcG) proteins assemble into multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes throughout development (1) . PHC3 (Polyhomeotic-like protein 3), also known as Early development regulatory protein 3, is a 983 amino acid nuclear protein that is a component of the PcG multiprotein PRC1 complex (2) . PRC1 complex acts via chromatin remodeling and modification of histones. PHC3 contains 1 FCS-type zinc finger and SAM (sterile alpha motif) domain (2-3) . PHC 3 has been regarded as a candidate tumor suppressor of osteosarcoma (4) .

NCBI Gene ID

80012

Swiss Prot

Q8NDX5

Accession Number

NP_079223

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Stem Cell

Purification

PHC3 antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1282 - 3T3 (NIH) Cell Lysate

Concentration

1 mg/mL

Buffer

PHC3 antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

PHC3 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

Fragment

IgG

Specificity

PHC3 antibody is human, mouse and rat specific. At least six isoforms of PHC3 are known to exist.

Symbol

PHC3

NCBI Official Name

Polyhomeotic homolog 3 (Drosophila)

NCBI Organism

Homo sapiens

Background Reference 01

Valk-Lingbeek ME, Bruggeman SW, and Van Lohuizen M. Stem cells and cancer; the polycomb connection. Cell 2004; 118:409–18.

Background Reference 02

Deshpande AM, Akunowicz JD, Reveles XT, et al. PHC3, a component of the hPRC-H complex, associates with E2F6 during G0 and is lost in osteosarcoma tumors. Oncogene 2007; 26:1714-22.

Background Reference 03

Isono K, Fujimura Y, Shinga J, et al. Mammalian polyhomeotic homologues Phc2 and Phc1 act in synergy to mediate polycomb repression of Hox genes. Mol. Cell Biol. 2005; 25:6694-706.

Background Reference 04

Iwata S, Takenobu H, Kageyama H, et al. Polycomb group molecule PHC3 regulates polycomb complex composition and prognosis of osteosarcoma. Cancer Sci. 2010; 101:1646-52.