MAP1LC3B Antibody

Product Specifications

Background

Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and plasticity (1) . MAP1LC3B belongs to the MAP1 LC3 family and it includes 3 different light chains, LC1, LC2 and LC3 (2) . MAP1LC3B is involved in formation of autophagosomal vacuoles (autophagosomes) (3) . It is most abundant in heart, brain, skeletal muscle and testis. MAP1LC3B is essential for autophagy and associated to the autophagosome membranes after processing (4) .

NCBI Gene ID

81631

Swiss Prot

Q9GZQ8

Accession Number

NP_073729

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Autophagy

Purification

MAP1LC3B antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1303 - Human Brain Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Bovine: (100%)

Buffer

MAP1LC3B antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

MAP1LC3B antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

Fragment

IgG

Specificity

MAP1LC3B antibody is human, mouse and rat reactive. Multiple isoforms MAP1LC3B are known to exist. MAP1LC3B antibody is predicted to not cross-react with MAP1LC3A or MAP1LC3C

Symbol

MAP1LC3B

Positive Control 2

Cat. No. 10-301 - Human Brain Tissue Slide

NCBI Official Name

Microtubule-associated protein 1 light chain 3 beta

NCBI Organism

Homo sapiens

Background Reference 01

Mandelkow E and Mandelkow EM. Microtubules and microtubule-associated proteins. Curr. Opin. Cell Biol. 1995; 7:72-81.

Background Reference 02

Fink JK, Jones SM, Esposito C, et al. Human microtubule-associated protein 1A (MAP1A) gene: genomic organization, cDNA sequence, and developmental and tissue-specific expression. Genomics 1996; 35:577-85.

Background Reference 03

Colecchia D, Strambi A, Sanzone S, et al. MAPK15/ERK8 stimulates autophagy by interacting with LC3 and GABARAP proteins. Autophagy 2012; 8:1724-40.

Background Reference 04

Kabeya Y, Mizushima N, Ueno T, et al. LC3, a mammalian homolog of yeast Apg8p, is localized in autophagosome membrane after processing. EMBO J. 2000; 19:5720-8.