IL-36B Antibody

Product Specifications

Background

IL-36B is is a member of the interleukin 1 cytokine family whose gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2 (1) . IL-36B is thought to activate the NF-kappaB pathway through IL-1 receptor family members IL-1RL2 and IL-1RAcP (2) . Like the related proteins IL-36A and IL-36G, IL-36B requires post-translational processing for full agonist activity, but the cleavage mechanism is currently unknown (3) . The IL-36 cytokines have been suggested to amplify Th1 responses by enhancing proliferation and Th1 polarization of naive CD4+ T cells (4) .

NCBI Gene ID

27177

Swiss Prot

Q9NZH7

Accession Number

NP_055253

Host

Rabbit

Reactivity

Human

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Immunology

Purification

IL-36B antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1203 - A549 Cell Lysate

Concentration

1 mg/mL

Buffer

IL-36B antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

IL-36B antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

Fragment

IgG

Specificity

IL-36B antibody is human specific. At least two isoforms of IL-36B are known to exist; this antibody will only detect the longer isoform. IL-36B antibody will not cross-react with IL-36A or IL-36G.

Symbol

IL36B

Positive Control 2

Cat. No. 10-101 - Human Lung Tissue Slide

NCBI Official Name

Interleukin 36, beta

NCBI Organism

Homo sapiens

Background Reference 01

Smith DE, Renshaw BR, Ketchem RR, et al. Four new members expand the interleukin-1 superfamily. J. Biol. Chem. 2000; 275:1169-75.

Background Reference 02

Towne JE, Garka KE, Renshaw BR, et al. Interleukin (IL) -1F6, IL-1F8, and IL-1F9 signal through IL-1Rrp2 and IL-1RAcP to activate the pathway leading to NF-kappaB and MAPKs. J. Biol. Chem. 2004; 279:13677-88.

Background Reference 03

Towne JE, Renshaw BR, Douangpanya J, et al. Interleukin-36 (IL-36) ligands require processing for full agonist agonist (IL-36a, IL-36b, and IL-36g) or antagonist (IL-36Ra) activity. J. Biol. Chem. 2011; 286:42594-602.

Background Reference 04

Vigne S, Palmer G, Martin P, et al. IL-36 signaling amplifies Th1 responses by enhancing proliferation and Th1 polarization of naive CD4+ T cells. Blood 2012; 120:3478-87.