FLI1 Antibody

Product Specifications

Background

The Friend leukemia virus integration 1 (FLI1) protein is a transcription factor containing an ETS DNA-binding domain and is highly expressed in hematopoietic cell lineages and vascular endothelial cells (1) . FLI1 plays important roles in megakaryocytic differentiation (2) and vascular homeostasis (3) . The FLI1 gene can undergo a t (11;22) (q24; q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. (4) . Abnormal expression of FLI1 can be used as an adverse prognostic indicator for acute myeloid leukemia (5) .

NCBI Gene ID

2313

Swiss Prot

Q01543

Accession Number

NP_002008

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Stem Cell

Purification

FLI1 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1205 - Jurkat Cell Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Bovine: (100%)

Buffer

FLI1 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

FLI1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

Fragment

IgG

Specificity

FLI1 antibody is human, mouse and rat reactive. At least four isoforms of FLI1 are known to exist; this antibody will detect all four.

Symbol

FLI1

Positive Control 2

Cat. No. 17-001 - HeLa Cell Slide

NCBI Official Name

Fli-1 proto-oncogene, ETS transcription factor

NCBI Organism

Homo sapiens

Background Reference 01

Hollenhorst PC, Jones DA, and Graves BJ. Expression profiles frame the promoter specificity dilemma of the ETS family of transcription factors. Nuc. Acids Res. 2004; 32:5693-702.

Background Reference 02

Spyropoulos DD, Pharr PN, Lavenburg KR, et al. Hemorrhage, impaired hematopoiesis, and lethality in mouse embryos carrying a targeted disruption of the Fli1 transcription factor. Mol. Cell Biol. 2000; 20:5643-52.

Background Reference 03

Asano Y, Stawski L, Hant F, et al. Endothelial Fli1 deficiency impairs vascular homeostasis: a role in scleroderma vasculopathy. Am. J. Pathol. 2010; 176:1983-98.

Background Reference 04

May WA, Gishizky ML, Lessnick SL, et al. Ewing sarcoma 11;22 translocation produces a chimeric transcription factor that requires the DNA-binding domain encoded by FLI1 for transformation. Proc. Natl. Acad. Sci. USA 1993; 90:5752-6. 5. Kornblau SM, Qiu YH, Zhang N, et al. Abnormal expression of FLI1 protein is an adverse prognostic factor in acute myeloid leukemia. Blood 2011; 118:5604-12.