SPP1 Antibody

Product Specifications

Background

SPP1 Antibody: The secreted protein 1 (SPP1), also known as osteopontin, is a major noncollagenous protein of bone, but is also found in the extracellular matrix of other mineralized tissues and in bodily fluids. In bone, SPP1 is produced by osteoblasts, osteocytes, macrophages, and osteoclasts (1,2) . SPP1 binds to cells through integrin and non-integrin receptors, as well as the adhesion receptor CD44 in an RGD-independent manner, enabling SPP1 to induce a number of functional effects including macrophage chemotaxis, cytoprotection, and regulation of T helper type 1 cells (2) . SPP1 can regulate biomineralization by inhibiting the formation of hydroxyapatite (3) and the growth of calcium oxalate crystals (4) .

NCBI Gene ID

6696

Swiss Prot

P10451

Accession Number

NP_001238759

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Stem Cell

Purification

SPP1 Antibody is Protein A purified.

Positive Control

Cat. No. 1310 - Human Bladder Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Pig: (94%), Bovine: (83%)

Buffer

SPP1 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

SPP1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

Fragment

IgG

Specificity

SPP1 antibody is human, mouse and rat reactive. Multiple isoforms of SPP1 are known to exist.

Symbol

SPP1

NCBI Official Name

Secreted phosphoprotein 1

NCBI Organism

Homo sapiens

Background Reference 01

Heinegard D, Andersson G, and Reinholt FP. Roles of osteopontin in bone remodeling. Ann. N.Y. Acad. Sci.1995; 760:213-22.

Background Reference 02

Wang KX and Denhardt DT. Osteopontin: rule in immune regulation and stress responses. Cyto. Growth Factor Rev. 2008; 19:333-45.

Background Reference 03

Boskey AL, Maresca M, Ullrich W, et al. Osteopontin-hydroxyapatite interactions in vitro: inhibition of hydroxyapatite formation and growth in a gelatin-gel. Bone Miner. 1993; 22:147-159.

Background Reference 04

Shiraga H, Min W, VanDusen WJ, et al. Inhibition of calcium oxalate crystal growth in vitro by uropontin: another member of the aspartic acid-rich protein superfamily. Proc. Natl. Acad. Sci. USA 1992; 89:426-30.