NCBP1 Antibody

Product Specifications

Background

NCBP1 Antibody: NCBP1, also known as CBP80, is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. NCBP1 promotes high-affinity mRNA-cap binding and associates with the CTD of RNA polymerase II. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay (1,2) . Recent evidence has shown that cellular-cap-binding proteins such as NCBP1 associate with influenza virus mRNAs, suggesting that these viral mRNAs may follow the normal cellular pathways for splicing, nuclear export, and translation (3) .

NCBI Gene ID

4686

Swiss Prot

Q09161

Accession Number

NP_002477

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Homeostasis

Purification

NCBP1 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1201 - HeLa Cell Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Chicken: (81%)

Buffer

NCBP1 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

NCBP1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

Fragment

IgG

Specificity

NCBP1 antibody is human, mouse and rat reactive. At least two isoforms of NCBP1 are known to exist; this antibody will detect both isoforms of NCBP1.

Symbol

NCBP1

Positive Control 2

Cat. No. 17-001 - HeLa Cell Slide

NCBI Official Name

Nuclear cap binding protein subunit 1, 80kDa

NCBI Organism

Homo sapiens

Background Reference 01

Ohno M, Kataoka N, and Shimura Y. A nuclear cap binding protein from HeLa cells. Nuc. Acid Res. 1990; 18:6989-95.

Background Reference 02

Maquat LE, Hwang J, Sato H, et al. CBP80-promoted mRNP rearrangements during the pioneer round of translation, nonsense-mediated mRNA decay, and thereafter. Cold Spring Harbor Symp. Quant. Biol. 2010; 75:127-34.