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DAP Antibody

Product Specifications

Background

DAP Antibody: The Death-associated protein (DAP) is a basic proline-rich 15kDa protein that as a positive mediator of programmed cell death that is induced by interferon-gamma (1) . DAP is also a direct substrate of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy. Under rich nutrient conditions, mTOR phosphorylates DAP at Ser3 and Ser51; under starvation conditions, these residues are dephosphorylated and DAP is converted into an active suppressor of autophagy (2) .

NCBI Gene ID

1611

Swiss Prot

P51397

Accession Number

NP_004385

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Autophagy

Purification

DAP Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1308 - Human Small Intestine Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Bovine: (89%)

Buffer

DAP Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

DAP antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

Fragment

IgG

Specificity

DAP antibody is human, mouse and rat reactive.

Symbol

DAP

Positive Control 2

Cat. No. 11-801 - Human Small Intestine Tissue Slide

NCBI Official Name

Death-associated protein

NCBI Organism

Homo sapiens

Background Reference 01

Deiss LP, Feinstein E, Berissi H, et al. Identification of a novel serine/threonine kinase and a novel 15-kD protein as potential mediators of the gamma interferon-induced cell death. Genes Dev. 1995; 9:15-30.

Background Reference 02

Koren I, Reem E, and Kimchi A. DAP1, a novel substrate of mTOR, negatively regulates autophagy. Curr. Biol. 2010; 20:1093-8.

Other Product Names

DAP Antibody: DAP1, Death-associated protein 1, DAP-1

Tested Applications

ELISA, WB, IHC-P, IF

Protein ID

4758120

Physical Properties

Liquid

Available Sizes

Frequently Asked Questions

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