B-raf Antibody

Product Specifications

Background

B-raf Antibody: B-raf belongs to the raf/mil family of serine/threonine protein kinases and plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. The Ras/Raf/MEK/ERK and Ras/PI3K/PTEN/Akt pathways interact with each other to regulate growth and in some cases tumorigenesis. Mutations in B-raf have been associated with several cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung, leading to speculation on the possibility of B-raf as a therapeutic target for treating cancers. Mutations in this gene have also been associated with cardiofaciocutaneous syndrome (CFCS), a disease characterized by heart defects, mental retardation and a distinctive facial appearance.

NCBI Gene ID

673

Swiss Prot

P15056

Accession Number

P15056

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

7379

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Validation

Independent Antibody Validation in Cell lines (Figure 2) shows similar B-raf expression profile in human and mouse cell lines detected by two independent anti-B-raf antibodies that recognize different epitopes, 5777 against central domain and 7379 against a slight different central domain. B-raf proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines5777 7379

Field of Research

Cancer, Cell Cycle

Purification

B-raf Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1303 - Human Brain Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Rat: (100%), Chicken: (100%)

Buffer

B-raf Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

B-raf antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

Fragment

IgG

Specificity

This antibody will not cross-react with C-raf.

Isoform

Human B-raf has one isoform (766aa, 84kD) . Mouse B-raf has 2 isoforms, including isoform 1 (804aa, 89kD) and isoform 2 (329aa, 37kD) . Rat B-raf a has one isoform (712aa, 80kD) . 7379 can detect in human B-raf and mouse isoform 1.

Symbol

BRAF

Positive Control 2

Cat. No. 11-801 - Human Small Intestine Tissue Slide

Positive Control 3

Cat. No. 1224 – Daudi Cell Lysate

Positive Control 4

Cat. No. 1204 – K562 Cell Lysate

NCBI Official Name

V-raf murine sarcoma viral oncogene homolog B1

NCBI Organism

Homo sapiens

Background Reference 01

McCubrey et al. Biophys. Acta 2007; 1773:1263-84.

Background Reference 02

Madhunapantula and Robertsonet al. Cancer Res.2008; 68:5-8.

Background Reference 03

Sarkozy et al. Hum. Mutat.2009; 30:695-702.

Other Product Names

B-raf Antibody: NS7, BRAF1, RAFB1, B-RAF1, Proto-oncogene B-Raf

Tested Applications

ELISA, WB, IHC-P, IF

Protein ID

50403720

Physical Properties

Liquid

Available Sizes

Curated Selection

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