RNF20 Antibody

Product Specifications

Background

RNF20 Antibody: RNF20 is an E3 ubiquitin ligase that regulates chromosome structure by monoubiquitinating histone H2B. RNF20 selectively suppresses the expression of some genes such as several protooncogenes and growth-related genes, including many genes that are induced by epidermal growth factor by interfering with chromatin recruitment of transcription elongation factor SII (TFIIS) . In contrast, RNF20 also positively regulates the p53 tumor suppressor as well as numerous histone H2A and H2B genes. Together with the deubiquitinase USP44, RNF20 regulates stem cell differentiation by modulating H2B monoubiquitination.

NCBI Gene ID

56254

Swiss Prot

Q5VTR2

Accession Number

NP_062538

Host

Rabbit

Reactivity

Human, Mouse

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Stem Cell

Purification

RNF20 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1304 - Human Liver Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Bovine: (100%), Chicken: (89%)

Buffer

RNF20 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

RNF20 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

Fragment

IgG

Specificity

Multiple isoforms of RNF20 are known to exist.

Symbol

RNF20

Positive Control 2

Cat. No. 10-201 - Human Liver Tissue Slide

NCBI Official Name

Ring finger protein 20, E3 ubiquitin protein ligase

NCBI Organism

Homo sapiens

Background Reference 01

Zhu B, Zheng Y, Pham AD, et al. Monoubiquitination of human histone H2B: the factors involved and their roles in HOX gene regulation. Mol. Cell 2005; 20:601-11.

Background Reference 02

Shema E, Kim J, Roeder RG, et al. RNF20 inhibits TFIIS-facilitated transcriptional elongation to suppress pro-oncogenic gene expression. Mol Cell 2011; 42:477-88.

Background Reference 03

Shema E, Tirosh I, Aylon Y, et al. The histone H2B-specific ubiquitin ligase RNF20/hBRE1 acts as a putative tumor suppressor through selective regulation of gene expression. Genes Dev. 2008; 22:2664-76.

Background Reference 04

Fuchs G, Shema E, Vesterman R, et al. RNF20 and USP44 regulate stem cell differentiation by modulating H2B monoubiquitylation. Mol. Cell 2012; 46:662-73.