ULK2 Antibody

Product Specifications

Background

ULK2 Antibody: ULK2, also known as ATG1B, is a key serine/threonine protein kinase probably acting at the most upstream step of autophagosome formation. Knockout of ULK2 results in a severe defect in the autophagy pathway. ULK2 is highly conserved among eukaryotes and shows high homology with its related protein ULK1. Both ULK1 and ULK2 form a complex with ATG13 and FIP200 that mediates TOR signaling and is essential for autophagy. Like ULK1, ULK2 is also thought to be involved in early neuronal growth and differentiation.

NCBI Gene ID

9706

Swiss Prot

Q8IYT8

Accession Number

NP_055498

Host

Rabbit

Reactivity

Human

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Autophagy

Purification

ULK2 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1303 - Human Brain Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Mouse: (82%)

Buffer

ULK2 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

ULK2 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

Fragment

IgG

Specificity

At least two isoforms of ULK2 are known to exist; this antibody will detect both isoforms. ULK2 antibody is predicted to not cross-react with ULK1.

Symbol

ULK2

Positive Control 2

Cat. No. 10-301 - Human Brain Tissue Slide

NCBI Official Name

Unc-51-like kinase 2 (C. elegans)

NCBI Organism

Homo sapiens

Background Reference 01

Suzuki K, Kubota Y, Sekito T, et al. Hierarchy of Atg proteins in pre-autophagosomal structure organization. Genes to Cells 2007; 12:209–18.

Background Reference 02

Lee EJ and Tournier C. The requirement of uncoordinated 51-like kinase 1 (ULK1) and ULK2 in the regulation of autophagy. Autophagy 2011; 7:689-95.

Background Reference 03

Jung CH, Jun CB, Ro SH, et al. ULK-ATG13-FIP200 complexes mediate mTOR signaling to the autophagy machinery. Mol. Biol. Cell 2009; 20:1992-2003.

Background Reference 04

Zhou X, Babu JR, da Silva S, et al.Unc-51-like kinase 1/2-mediated endocytic processes regulate filopodia extension and branching of sensory axons. Proc. Natl. Acad. Sci. USA 2007; 104:5842-7.