SHISA3 Antibody

Product Specifications

Background

SHISA3 Antibody: SHISA3 plays an essential role in the maturation of presomitic mesoderm cells by individual attenuation of both FGF and WNT signaling. The Shisa family of single-transmembrane proteins is characterized by an N-terminal cysteine-rich domain and a proline-rich C-terminal region. Its founding member, Xenopus Shisa, promotes head development by antagonizing Wnt and FGF signaling. Shisa physically interacted with immature forms of the Wnt receptor Frizzled and the FGF receptor within the ER and inhibited their posttranslational maturation and trafficking to the cell surface. Loss of Shisa function sensitized the neuroectoderm to Wnt signaling and suppressed head formation during gastrulation.

NCBI Gene ID

152573

Swiss Prot

A0PJX4

Accession Number

NP_001073974

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Stem Cell

Purification

SHISA3 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1308 - Human Small Intestine Tissue Lysate

Concentration

1 mg/mL

Buffer

SHISA3 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

SHISA3 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

Fragment

IgG

Specificity

SHISA3 antibody is predicted to not cross-react with other SHISA protein family members.

Symbol

SHISA3

Positive Control 2

Cat. No. 11-801 - Human Small Intestine Tissue Slide

NCBI Official Name

Shisa homolog 3 (Xenopus laevis)

NCBI Organism

Homo sapiens

Background Reference 01

Furushima K, Yamamoto A, Nagano T, et al. Mouse homologues of Shisa antagonistic to Wnt and Fgf signalings. Dev. Biol. 2007; 306:480-92.

Background Reference 02

Pei J and Grishin NV. Unexpected diversity in Shisa-like proteins suggests the importance of their roles as transmembrane adaptors. Cell Signal. 2012; 24:758-69.

Background Reference 03

Yamamoto A, Nagano T, Takehara S, et al. Shisa promotes head formation through the inhibition of receptor protein maturation for the caudalizing factors, Wnt and FGF. Cell 2005; 120:223-35