CRB2 Antibody

Product Specifications

Background

CRB2 Antibody: CRB2 (Crumbs homolog 2), like its homologs CRB1 and CRB3, is similar to the Drosophila crumbs protein and is expressed in retina, brain and kidney. Along with other proteins, the Crumbs proteins form a complex that help set up cell polarity in developing neuroepithelial cells. At the onset of neural specification, embryonic stem cells (ESCs) upregulate CRB2, which then localizes apically in neural rosettes. Gain- and loss-of-function studies of CRB2 have shown that CRB2 is essential for the stabilization of other polarity proteins. Unlike CRB1, mutations in CRB2 do not appear to play a role in retinitis pigmentosa or in Leber congenital amaurosis.

NCBI Gene ID

286204

Swiss Prot

Q5IJ48

Accession Number

NP_775960

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Stem Cell

Purification

CRB2 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1463 - Rat Brain Tissue Lysate

Concentration

1 mg/mL

Buffer

CRB2 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

CRB2 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.

Fragment

IgG

Specificity

At least three isoforms of CRB2 are known to exist; this antibody will detect all three. CRB2 antibody is predicted to not cross-react with other members of the CRB family.

Symbol

CRB2

Positive Control 2

Cat. No. 1305 - Human Kidney Tissue Lysate

Positive Control 3

Cat. No. 1403 - Mouse Brain Tissue Lysate

NCBI Official Name

Crumbs homolog 2 (Drosophila)

NCBI Organism

Homo sapiens

Background Reference 01

Van den Hurk J, Rashbass P, Roepman R, et al. Characterization of the Crumbs homolog 2 (CRB2) gene and analysis of its role in retinits pigmentosa and Leber congenital amaurosis. Mol. Viso. 2005; 11:263-73

Background Reference 02

Bulgakova NA and Knust E. The Crumbs complex: from epithelial-cell polarity to retinal degeneration. J. Cell Sci. 2009; 122:2587-96.

Background Reference 03

Boroviak T and Rashbass P. The apical polarity determinant Crumbs 2 is a novel regulator of ESC-derived neural progenitors. Stem Cells 2011; 29:193-205