LMX1B Antibody

Product Specifications

Background

LMX1B Antibody: The LIM homeobox transcription factor 1B (LMX1B) belongs to the LIM-homeodomain family. Members of this family are known to be important for pattern formation during development. LMX1B regulates mid-hindbrain patterning; LMX1B-null mice embryos have a severe reduction in the number of midbrain dopaminergic neurons compared to wild-type. While LMX1B appears to be important for both the development and the survival of dopamine neurons, the related LMX1A is crucial for the differentiation of these cells. However, LMX1A and LMX1B function cooperatively to regulated the proliferation, specification and differentiation of midbrain dopaminergic neuronal progenitors.

NCBI Gene ID

4010

Swiss Prot

O60663

Accession Number

NP_001167617

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Stem Cell

Purification

LMX1B Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1288 - A20 Cell Lysate

Concentration

1 mg/mL

Buffer

LMX1B Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

LMX1B antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

45 kDa

Fragment

IgG

Specificity

At least three isoforms are known to exist. This antibody is predicted to not cross-react with LMX1A.

Symbol

LMX1B

Positive Control 2

Cat. No. 10-301 - Human Brain Tissue Slide

NCBI Official Name

LIM homeobox transcription factor 1, beta

NCBI Organism

Homo sapiens

Background Reference 01

Hobert O and Westphal H. Functions of LIM-homeobox genes. Trends Genet. 2000; 16:75-83.

Background Reference 02

Nakatani T, Kumai M, Mizuhara E, et al. Lmx1a and Lmx1b cooperate with Foxa2 to coordinate the specification of dopaminergic neurons and control of floor plate cell differentiation in the developing mesencephalon. Dev. Biol. 2010; 339:101-13.

Background Reference 03

Guo C, Qiu HY, Huang Y, et al. Lmx1b is essential for FGF8 and Wnt1 expression in the isthmic organizer during tectum and cerebellum development in mice. Development 2007; 134:317-25.