Pellino 1 Antibody

Product Specifications

Background

Pellino 1 Antibody: The Pellino proteins are a highly homologous family of E3 ubiquitin ligases that act as upstream mediators in Toll-like receptor (TLR) pathways that lead to activation of MAP kinases and transcription factors. Pellino 1 is required for interleukin-1-mediated signaling through its interaction with the IRAK4-IRAK-TRAF6 complex, ultimately resulting in the activation of NF-κ B. Like other members of the Pellino family, Pellino 1 is an E3 ubiquitin ligase, able to catalyze the polyubiquitination of IRAK1. It is activated via phosphorylation by either IRAK1 and IRAK4 or the IKK-related kinases IKK-ε and TBK1. In addition to phosphorylation, Pellino 1 activity is also modulated via ubiquitination and sumoylation.

NCBI Gene ID

57162

Swiss Prot

Q96FA3

Accession Number

NP_065702

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Innate Immunity

Purification

Pellino 1 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1304 - Human Liver Tissue Lysate

Concentration

1 mg/mL

Buffer

Pellino 1 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Pellino 1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

46 kDa

Fragment

IgG

Symbol

PELI1

Positive Control 2

Cat. No. 17-011 - Hep G2 Cell Slide

NCBI Official Name

Pellino E3 ubiquitin protein ligase 1

NCBI Organism

Homo sapiens

Background Reference 01

Moynagh PN. The Pellino family: IRAK E3 ligases with emerging roles in innate immune signaling. Trends Immunol. 2009; 30:33-42.

Background Reference 02

Jiang Z, Johnson J, Nie H, et al. Pellino 1 is required for interleukin-1 (IL-1) -mediated signaling through its interaction with the IL-1 receptor-associated kinase 4 (IRAK4) -IRAK-tumor necrosis factor receptor-associated factor 6 (TRAF6) complex. J. Biol. Chem. 2003; 278:10952-6

Background Reference 03

Butler MP, Hanly JA, and Moynagh PN. Kinase-active interleukin-1 receptor-associated kinases promote polyubiquitination and degradation of the Pellino family: direct evidence for Pellino proteins being ubiquitin-protein isopeptide ligases. J. Biol. Chem. 2007; 282:29729-37.

Background Reference 04

Goh ET, Arthur JS, Cheung PC, et al. Identification of the protein kinases that activate the E3 ubiquitin ligase Pellino 1 in the innate immune system. Biochem. J. 2012; 441:339-46