CITED2 Antibody

Product Specifications

Background

CITED2 Antibody: CITED2 was initially identified as a potential transcriptional activator with significant homology to MSG1, a melanocyte-specific nuclear protein. CITED2 interacts with the histone acetyltransferase p300/CBP and acts as a coactivator to several DNA-binding transcription factors such as HIF-1 and AP-2. CITED2 also controls proliferation of mouse embryonic fibroblasts via the polycomb group genes Bmi-1 and Mel18 and the tumor suppressor Ink4a/Arf. CITED2 has also been found to be an essential regulator of adult hematopoietic stem cells (HSCs), with conditional deletion of CITED2 in the adult mouse resulting in the loss of HSCs, multilineage bone marrow failure and increased lethality.

NCBI Gene ID

10370

Swiss Prot

Q99967

Accession Number

NP_001161861

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Stem Cell

Purification

CITED2 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1205 - Jurkat Cell Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Bovine: (100%)

Buffer

CITED2 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

CITED2 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

30 kDa

Fragment

IgG

Specificity

Multiple isoforms of CITED2 are known to exist.

Symbol

CITED2

Positive Control 2

Cat. No. 17-005 - Jurkat Cell Slide

NCBI Official Name

Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 2

NCBI Organism

Homo sapiens

Background Reference 01

Shioda T, Fenner MH, and Isselbacher KJ. MSG1 and its related protein MRG1 share a transcription activating domain. Gene 1997; 204:235-41.

Background Reference 02

Bhattachatya S, Michels CL, Leung MK, et al. Functional role of p35srj, a novel p300/CBP binding protein, during transactivation by HIF-1. Genes Dev. 1999; 13:64-75.

Background Reference 03

Bamforth SD, Braganca J, Eloranta JJ, et al. Cardiac malformations, adrenal agenesis, neural crest defects and exencephaly in mice lacking Cited2, a new Tfap2 co-activator. Nat. Genet. 2001; 29:469-74.

Background Reference 04

Kranc KR, Bamforth SD, Braganca J, et al. Transcriptional coactivator Cited2 induces Bmi1 and Mel18 and controls fibroblast proliferation via Ink4a/ARF. Mol. Cell. Biol. 2003; 23:7658-66.