PLA1A Antibody

Product Specifications

Background

PLA1A Antibody: PLA1A is a phospholipase that hydrolyzes fatty acids at the sn-1 position of phosphatidylserine and 1-acyl-2-lysophosphatidylserine. This secreted protein hydrolyzes phosphatidylserine (PS) in liposomes and can also hydrolyze PS in apoptotic cells and activate platelets where the resulting 2-acyl-lysophosphatidylserine acts as a lipid mediator for mast cells, T cells, and neural cells, suggesting that a major function of PLA1A may be the production of lysophospholipid mediators. PLA1A is upregulated in rat peripheral blood cells bearing long-term surviving cardiac allograft. PLA1A is also expressed in human THP-1-derived macrophages and this expression is upregulated in cells treated with lipopolysaccharide, a TLR4 ligand. This upregulation is inhibited with corticosteroids, which are often used at high dosages to suppress chronic allograft rejection.

NCBI Gene ID

51365

Swiss Prot

Q53H76

Accession Number

NP_056984

Host

Rabbit

Reactivity

Human, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Immunology

Purification

PLA1A Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1305 - Human Kidney Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Mouse: (75%), Bovine: (71%)

Buffer

PLA1A Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

PLA1A antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

50 kDa

Fragment

IgG

Specificity

At least three isoforms of PLA1A are known to exist; this antibody will detect all three isoforms.

Symbol

PLA1A

Positive Control 2

Cat. No. 10-401 - Human Kidney Tissue Slide

NCBI Official Name

Phospholipase A1 member A

NCBI Organism

Homo sapiens

Background Reference 01

Sato T, Aoki J, Nagai Y, et al. Serine phospholipid-specific phospholipase A that is secreted from activated platelets. A new member of the lipase family. J. Biol. Chem. 1997; 272:2192-8.

Background Reference 02

Aoki J, Nagai Y, Hosono H, et al. Structure and function of phosphatidylserine-specific phospholipase A1. Biochim. Biophys. Acta 2002; 1582:26-32.

Background Reference 03

Hosono H, Homma M, Ogasawa Y, et al. Expression of phosphatidylserine-specific phospholipase A (1) mRNA in human THP-1-derived macrophages. Cell Transplant. 2010; 19:759-64.