ATG14 Antibody

Product Specifications

Background

ATG14 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein ATG1. ATG14 is a subunit of a class III phosphatidylinositol (PtdIns) 3-kinase complex that targets the complex to the endoplasmic reticulum, the site of autophagosome formation.

NCBI Gene ID

22863

Swiss Prot

Q6ZNE5

Accession Number

NP_055739

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Autophagy

Purification

ATG14 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1308 - Human Small Intestine Tissue Lysate

Concentration

1 mg/mL

Buffer

ATG14 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

ATG14 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

54 kDa

Fragment

IgG

Specificity

At least three isoforms of ATG14 are known to exist; this antibody will detect all three isoforms

Symbol

ATG14

Positive Control 2

Cat. No. 11-801 - Human Small Intestine Tissue Slide

NCBI Official Name

ATG14 autophagy related 14 homolog (S. cerevisiae)

NCBI Organism

Homo sapiens

Background Reference 01

Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene 2004; 23:2891-906.

Background Reference 02

Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.

Background Reference 03

Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol. 2000; 150:1507-13.

Background Reference 04

Itakura E, Kishi C, Inoue K, et al. Beclin 1 forms two distinct phosphatidylinositol 3-kinase complexes with mammalian Atg14 and UVRAG. Mol. Biol. Cell 2008; 19:5360-72.