WIPI1 Antibody

Product Specifications

Background

WIPI1 Antibody: WIPI1 (WD repeat domain, phosphoinositide interacting-1), also known as WIPI1, ATG18 or WIPI49, is thought to play a role in autophagy and may regulate protein trafficking in certain recycling pathways. It contains three WD repeats and has a 7-bladed propeller structure with a conserved motif that facilitates its interaction with other proteins. WIPI1 localizes to cytoplasmic vesicles, endosomes, clathrin-coated vesicles and the trans-Golgi network. It is ubiquitously expressed with highest expression in heart, testis, placenta, pancreas and skeletal muscle. WIPI1 is upregulated in a variety of tumors, suggesting a role in carcinogenesis.

NCBI Gene ID

55062

Swiss Prot

Q5MNZ9

Accession Number

NP_060453

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Autophagy

Purification

WIPI1 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1472 - Rat Colon Tissue Lysate

Concentration

1 mg/mL

Buffer

WIPI1 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

WIPI1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

49 kDa

Fragment

IgG

Specificity

At least two isoforms of WIPI1 are known to exist; this antibody will detect both isoforms.

Symbol

WIPI1

NCBI Official Name

WD repeat domain, phosphoinositide interacting 1

NCBI Organism

Homo sapiens

Background Reference 01

Proikas-Cezanne T and Robenek H. Freeze-fracture replica immunolabelling reveals human WIPI-1 and WIPI-2 as membrane proteins of autophagosomes. J. Cell. Mol. Med. 2011; 15:2007-10.

Background Reference 02

Jeffries TR, Dove SK, Michell RH, et al. PtdIns-specific MPR pathway association of a novel WD40 repeat protein, WIPI49. Mol. Biol. Cell. 2004;15:2652-63.

Background Reference 03

Proikas-Cezanne T, Waddell S, Gaugel A, et al. WIPI-1 (WIPI49), a member of the novel 7-bladed WIPI protein family, is aberrantly expressed in human cancer and is linked to starvation-induced autophagy. Oncogene 2004; 23:9314-25.

Background Reference 04

Proikas-Cezanne T, Ruckerbauer S, Stierhof YD, et al. Human WIPI-1 puncta-formation: a novel assay to assess mammalian autophagy. FEBS Lett. 2007; 581:3396-404.