FLT3 Antibody

Product Specifications

Background

FLT3 Antibody: FLT3 is a member of the class III receptor tyrosine kinase family and its internal tandem duplication (ITD) is preferentially seen in acute myeloid leukemia and myelodysplastic syndrome among various hematological malignancies. It is a membrane bound glycosylated protein that is expressed primarily in early hematopoietic progenitor cells. Upon binding its ligand FL, FLT3 activates multiple signaling pathways including the PI3K/Akt and Ras/MEK/MAPK pathways. In cells expressing FLT3-ITD, these pathways are constitutively on, resulting in abnormal cell growth and anti-apoptotic activity.

NCBI Gene ID

2322

Swiss Prot

P36888

Accession Number

NP_004110

Host

Rabbit

Reactivity

Human, Mouse

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Stem Cell

Purification

FLT3 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1212 - 3T3 Cell Lysate

Concentration

1 mg/mL

Buffer

FLT3 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

FLT3 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

109 kDa

Fragment

IgG

Specificity

At least three isoforms of FLT3 are known to exist; this antibody will detect all isoforms.

Symbol

FLT3

Positive Control 2

Cat. No. 10-401 - Human Kidney Tissue Slide

NCBI Official Name

Fms-related tyrosine kinase 3

NCBI Organism

Homo sapiens

Background Reference 01

Rosnet O, Schiff C, Pebusque MJ, et al. Human FLT3/FLK2 gene: cDNA cloning and expression in hematopoietic cells. Blood 1993; 82:1110-9

Background Reference 02

Yokoa S, Kiyoi H, Nakao M, et al. Internal tandem duplication of the FLT3 gene is preferentially seen in acute myeloid leukemia and myelodysplastic syndrome among various hematological malignancies. Leukemia 1997; 11:1605-9.

Background Reference 03

Takahashi S. Downstream molecular pathways of FLT3 in the pathogenesis of acute myeloid leukemia: biology and therapeutic implications. J. Hematol. and Oncol. 2011; 4:13.