EED Antibody

Product Specifications

Background

EED Antibody: The EED protein (WAIT-1 or WD protein associated with integrin cytoplasmic tails-1), also called embryonic ectoderm development, is a member of the superfamily of WD-40 repeat proteins and widely conserved polycomb group (PcG) family of proteins. The polycomb group (PcG) is a large and evolutionarily conserved set of genes whose products act in multimeric complexes to modify histones, which are then thought to cause stable and heritable states of transcriptional repression. EED shuttles between the nucleus and the plasma membrane and can interact with the cytoplasmic tail of integrin β7 subunit. EED exerted an antiviral activity at the late stage of HIV-1 replication.

NCBI Gene ID

8726

Swiss Prot

O75530

Accession Number

NP_003788

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Stem Cell

Purification

EED Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1313 - Human Testis Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Chicken: (100%), Bovine: (100%)

Buffer

EED Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

EED antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Specificity

Four alternatively spliced transcript variants have been observed.

Symbol

EED

Positive Control 2

Cat. No. 10-501 - Human Heart Tissue Slide

NCBI Official Name

Embryonic ectoderm development

NCBI Organism

Homo sapiens

Background Reference 01

Rietzler M, Bittner M, Kolanus W, et al. The human WD repeat protein WAIT-1 specifically interacts with the cytoplasmic tails of beta7-integrins. J. Biol. Chem. 1998; 273:27459-66.

Background Reference 02

Brock HW and Fisher CL. Maintenance of gene expression patterns. Dev Dyn. 2005; 232:633-55.

Background Reference 03

Levine SS, King IF, Kingston RE, et al. Division of labor in polycomb group repression. Trends Biochem. Sci. 2004; 29: 478-85.

Background Reference 04

Van der Vlag J and Otte AP. Transcriptional repression mediated by the human Polycomb-group protein EED involves histone deacetylation. Nat. Genet.1999; 23:474-8.