CBX4 Antibody

Product Specifications

Background

CBX4 Antibody: Polycomb group (PcG) proteins are chromatin-associated proteins which are important for embryonic and adult stem cell self-renewal and maintenance. At least two distinct human PcG complexes have been identified. Polycomb repressive complex I (PRC1) contains CBX4/Pc2, HPH1 and RING domain-containing proteins (RING1, RING2 and BMI1) . CBX4 (Chromobox protein homolog 4 or PC2), which functions as a transcriptional suppressor, is a candidate of KyoT2-binding proteins. It is a E3 SUMO-protein ligase which facilitates SUMO1 conjugation. Depletion of CBX4 results in decreased cellular resistance to ionizing radiation. CBX4 is directly involved in the cellular response to DNA damage.

NCBI Gene ID

8535

Swiss Prot

O00257

Accession Number

NP_003646

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Stem Cell

Purification

CBX4 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1303 - Human Brain Tissue Lysate

Concentration

1 mg/mL

Buffer

CBX4 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

CBX4 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

62 kDa

Fragment

IgG

Specificity

Two alternatively spliced transcript variants have been observed.

Symbol

CBX4

Positive Control 2

Cat. No. 10-301 - Human Brain Tissue Slide

NCBI Official Name

Chromobox homolog 4

NCBI Organism

Homo sapiens

Background Reference 01

Simon JA and Tamkun JW. Programming off and on states in chromatin: mechanisms of Polycomb and trithorax group complexes. Curr. Opin. Genet. Dev. 2002;12:210-8.

Background Reference 02

Gieni RS and Hendzel MJ. Polycomb group protein gene silencing, non-coding RNA, stem cells, and cancer. Biochem. Cell Biol. 2009; 87:711-46.

Background Reference 03

Luis NM, Morey L, Mejetta S, et al. Regulation of human epidermal stem cell proliferation and senescence requires polycomb-dependent and -independent functions of Cbx4. Cell. Stem Cell 2011; 9:233-46.

Background Reference 04

Ismail IH, Gagné JP, Caron MC, et al. CBX4-mediated SUMO modification regulates BMI1 recruitment at sites of DNA damage. Nucleic Acids Res. 2012 Mar 8.