TOX Antibody

Product Specifications

Background

TOX Antibody: TOX (thymocyte selection-associated high mobility group (HMG) box protein) is a member of the HMG box family of DNA-binding proteins and likely plays a role in the regulation of T-cell development. It is a 526 amino acid nuclear protein and the expression of TOX is upregulated by pre-T cell receptor (pre-TCR) and TCR activation in immature thymocytes. TOX-mediated positive selection is associated with up-regulation of RUNX3 and is calcineurin dependent. TOX-dependent transition to the CD4+CD8 stage is required for development of class II major histocompatibility complex-specific T cells.

NCBI Gene ID

9760

Swiss Prot

O94900

Accession Number

NP_055544

Host

Rabbit

Reactivity

Human

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Immunology

Purification

TOX Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1320 - Human Colon Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Mouse: (76%)

Buffer

TOX Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

TOX antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Calculated Molecular Weight

58 kDa

Fragment

IgG

Specificity

No alternatively spliced transcript variants have been observed.

Symbol

TOX

NCBI Official Name

Thymocyte selection-associated high mobility group box protein TOX

NCBI Organism

Homo sapiens

Background Reference 01

Wilkinson B, Chen JY, Han P, et al. TOX: an HMG box protein implicated in the regulation of thymocyte selection. Nat. Immunol. 2002; 3:272-80.

Background Reference 02

Saito T and Watanabe N. Positive and negative thymocyte selection. Crit. Rev. Immunol. 1998; 18:359-70

Background Reference 03

Aliahmad P, O'Flaherty E, Han P, et al. TOX provides a link between calcineurin activation and CD8 lineage commitment. J. Exp. Med. 2004; 199:1089-99.

Background Reference 04

Aliahmad P, Kadavallore A, de la Torre B, et al. TOX is required for development of the CD4 T cell lineage gene program. J. Immunol. 2011; 187:5931-40.