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Fat Free Antibody

Product Specifications

Background

Fat Free Antibody: Fat Free, also known as ANG2, was identified though a yeast two-hybrid screen using VP53. It is thought to be a structural and functional homolog of the yeast protein VPS51, a protein that together with VPS52, VPS53, and VPS54 form the Golgi-associated retrograde protein (GARP) complex that mediates the tethering and fusion of endosome-derived transport carriers to the trans-Golgi network (TGN) . Similar to depletion of any of the VPS52, VPS53, or VPS54, depletion of Fat Free using RNAi impairs protein retrieval to the TGN indicating that Fat Free is missing component of the GARP complex in most eukaryotes.

NCBI Gene ID

738

Swiss Prot

Q9UID3

Accession Number

NP_037397

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Homeostasis

Purification

Fat Free Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1403 - Mouse Brain Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Bovine: (100%), Chicken: (82%)

Buffer

Fat Free Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Fat Free antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=6979

Calculated Molecular Weight

86 kDa

Fragment

IgG

Specificity

At least two alternatively spliced isoforms are known to exist; this antibody will detect both isoforms.

Symbol

C11orf2

NCBI Official Name

Chromosome 11 open reading frame 2

NCBI Organism

Homo sapiens

Background Reference 01

Perez-Victoria FJ, Schindler C, Magadan JG, et al. Ang2/Fat Free is a conserved subunit of the Golgi-associated retrograde protein complex. Mol. Biol. Cell 2010; 21:3386-95

Background Reference 02

Ho SY, Lorent K, Pack M, et al. Zebrafish Fat Free is required for intestinal lipid absorption and Golgi apparatus structure. Cell Metab. 2006; 3:289-300.

Background Reference 03

Perez-Victoria FJ, Mardones GA, and Bonifacino JS. Requirement of the human GARP complex for mannose 6-phosphate-receptor-dependent sorting of cathepsin D to lysosomes. Mol. Biol. Cell 2008; 19:2350-62

Background Reference 04

Stafford RL, Ear J, Knight MJ, et al. The molecular basis of the Caskin1 and Mint1 interaction with CASK. J. Mol. Biol. 2011; 412:3-13.

Other Product Names

Fat Free Antibody: FFR, ANG2, ANG3, C11orf2, C11orf3, FFR, PP5382, Vacuolar protein sorting-associated protein 51 homolog, Another new gene 2 protein

Tested Applications

ELISA, WB

Protein ID

8393009

Physical Properties

Liquid

Available Sizes

Curated Selection

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