RAB21 Antibody

Product Specifications

Background

RAB21 Antibody: RAB21 is a member of a subfamily of small GTP-binding protein genes of the Ras superfamily that plays an important role in intracellular vesicular targeting. In non-polarized Caco-2 cells, RAB21 showed a general endoplasmic reticulum (ER) -like localization, while in polarized cells, RAB21 localized to apical vesicles. RAB21 has been shown to associate with integrin subunits and to be important for receptor entry into cells via RAB5/RAB21 endosomes. The GTPase-activating protein p120RasGAP regulates cell motility by controlling the return of the endocytosed integrins to the plasma membrane by competing with RAB21 for binding to overlapping sites on the α -tail of endocytosed integrin.

NCBI Gene ID

23011

Swiss Prot

Q9UL25

Accession Number

NP_055814

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Homeostasis

Purification

RAB21 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1405 - Mouse Kidney Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Bovine: (95%)

Buffer

RAB21 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

RAB21 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Specificity

This antibody is predicted to not cross-react with other Ras-related proteins

Symbol

RAB21

Positive Control 2

Cat. No. 10-401 - Human Kidney Tissue Slide

NCBI Official Name

RAB21, member RAS oncogene family

NCBI Organism

Homo sapiens

Background Reference 01

Opdam FJ, Kamps G, Croes H, et al. Expression of Rab small GTPases in epithelial Caco-2 cells: Rab21 is an apically located GTP-binding protein in polarised intestinal epithelial cells. Eur. J. Cell Biol. 2000; 79:308-16.

Background Reference 02

Pellinen T and Ivaska J. Integrin traffic. J. Cell Sci. 2006; 119:3723-31.

Background Reference 04

Mai A, Veltel S, Pellinen T, et al. Competitive binding of Rab21 and p120RasGAP to integrins regulates receptor traffic and migration. J. Cell Biol. 2011; 194:291-306.