SDHD Antibody

Product Specifications

Background

SDHD Antibody: The mitochondrial succinate dehydrogenase complex subunit D (SDHD) is one of four proteins that make up the tricarboxylic cycle enzyme succinate dehydrogenase (SCH) . Studies have shown that mutations in SDHD often leads to hereditary paragangliomas, usually benign tumors of the autonomic nervous system, suggesting that SDHD also plays a role as a tumor-suppressor gene. In one family with a nonsense mutation (R22X) in the SDHD gene, a loss of heterozygosity was found in the paragangliomas, and within these tumors the enzymatic activity of Complex II in the mitochondrial respiratory chain was completely abolished. Furthermore, high levels of angiogenic factors EPAS1 and VEGF was observed, which may stimulate tumor growth.

NCBI Gene ID

9392

Swiss Prot

O14521

Accession Number

NP_002993

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Cell Cycle

Purification

SDHD Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1287 - EL4 Cell Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Bovine: (100%), Sheep: (100%), Pig: (80%)

Buffer

SDHD Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

SDHD antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Specificity

At least four isoforms of SDHD are known to exist; this antibody will detect the two longest isoforms.

Symbol

SDHD

Positive Control 2

Cat. No. 17-207 - EL4 Cell Slide

NCBI Official Name

Succinate dehydrogenase complex, subunit D, integral membrane protein

NCBI Organism

Homo sapiens

Background Reference 01

Baysal BE, Ferrell RE, Willett-Brozick JE, et al. Mutations in SDHD, a mitochondrial complex II gene, in hereditary paraganglioma. Science 2000; 287:848-51.

Background Reference 02

Saraste M. Oxidative phosphorylation at the fin de siecle. Science 1999; 283:1488-93.

Background Reference 03

Knudson AG. Genetics of human cancer. Annu. Rev. Genet. 1986; 20:231-51.

Background Reference 04

Gimenez-Roqueplo AP, Favier J, Rustin P, et al. The R22X mutation of the SDHD gene in hereditary paraganglioma abolishes the enzymatic activity of Complex II in the mitochondrial respiratory chain and activates the hypoxia pathway. Am. J. Hum. Genet. 2001; 69:1186-97.