GRB10 Antibody

Product Specifications

Background

GRB10 Antibody: GRB10 belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules and function in diverse cellular processes. GRB10 is a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. Overexpression of some isoforms of GRB10 inhibits tyrosine kinase activity and results in growth suppression. GRB10 can also interact with the L isoform of the proapoptotic protein Bim. This gene is imprinted in a highly isoform- and tissue-specific manner, with expression observed from the paternal allele in the brain, and from the maternal allele in the placental trophoblasts.

NCBI Gene ID

2887

Swiss Prot

Q13322

Accession Number

NP_005302

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Signal Transduction

Purification

GRB10 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1220 - SK-N-SH Cell Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Pig: (100%)

Buffer

GRB10 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

GRB10 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Specificity

At least three isoforms of GRB10 are known to exist; this antibody will detect all three isoforms.

Symbol

GRB10

NCBI Official Name

Growth factor receptor-bound protein 10

NCBI Organism

Homo sapiens

Background Reference 01

Morrione A. Grb10 proteins in insulin-like growth factor and insulin receptor signaling (review) . Int. J. Mol. Med. 2000; 5:151-4.

Background Reference 02

Liu F and Roth RA. Grb-IR: a SH2-domain-containing protein that binds to the insulin receptor and inhibits its function. Proc. Natl. Acad. Sci. USA 1995; 92:10287-91.

Background Reference 03

Wick KR, Werner ED, Langlais P, et al. Grb10 inhibits insulin-stimulated insulin receptor substrate (IRS) -phosphatidylinositol 3-kinase/Akt signaling pathway by disrupting the association of IRS-1/IRS-2 with the insulin receptor. J. Biol. Chem. 2003; 278:8460-7.