JMJD3 Antibody

Product Specifications

Background

JMJD3 Antibody: The Jumonji domain-containing protein 3 (JMJD3) functions as a trimethylation-specific demethylase, converting the trimethylated histone H3 Lys27 residue to the dimethylated form, and is thought to also function as a transcriptional repressor. JMJD3 plays a central role in regulation of posterior development, by regulating HOX gene expression. It is involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation. JMJD3 can also interact with and demethylate p53, resulting in its stabilization and localization to the nucleus in mouse embryo fibroblasts during neural stem cell differentiation.

NCBI Gene ID

23135

Swiss Prot

O15054

Accession Number

EAW90126

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

6657

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Validation

Independent Antibody Validation in Cell lines (Figure 2) shows similar JMJD3 expression profile in human, mouse and rat cell lines detected by two independent anti-IFN-beta antibodies that recognize different epitopes, 6657 against C-terminus domain and 14-618 against N-terminus domain. IFN-beta proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines6657 14-618

Field of Research

Stem Cell

Purification

JMJD3 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1201 - HeLa Cell Lysate

Concentration

1 mg/mL

Buffer

JMJD3 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

JMJD3 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Specificity

This antibody is specific for JMJD3 and will not recognize other JMJD proteins.

Isoform

Human JMJD3 has 2 isoforms, including isoform 1 (1682aa, 181kD) and isoform 2 (1643aa, 177kD) . Mouse JMJD3 has one isoform (1641aa, 176kD) and rat JMJD3 also has one isoform (1356aa, 146kD) . 6657 can detect human, mouse and rat.

Symbol

KDM6B

Positive Control 2

Cat. No. 17-004 - K-562 Cell Slide

Positive Control 3

Cat. No. 1204 - K562 Cell Lysate

Positive Control 4

Cat. No. 1288 - A-20 Cell Lysate

NCBI Official Name

Lysine (K) -specific demethylase 6B

NCBI Organism

Homo sapiens

Background Reference 01

Agger et al. Nature 2007; 449:731-4.

Background Reference 02

De Santa et al. Cell 2007; 130:1083-94.

Background Reference 03

Sola et al. PLoS One 2011; 6:e18421.

Other Product Names

JMJD3 Antibody: JMJD3, JMJD3, KIAA0346, Lysine-specific demethylase 6B, JmjC domain-containing protein 3

Tested Applications

ELISA, WB, ICC, IF

Protein ID

122937251

Physical Properties

Liquid

Available Sizes

Curated Selection

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