PRICKLE1 Antibody

Product Specifications

Background

PRICKLE1 Antibody: PRICKLE1, also known as RILP or EPM1B, is a Disheveled-associated protein that serves as a nuclear translocation receptor for REST/NRSF and REST4 (1, 2) . It contains three N-terminal LIM domains and three C-terminal nuclear localization signals. It localizes to the cytoplasm, as well as to the nuclear membrane and expressed at higher levels in placenta. PRICKLE1 is a negative regulator of the Wnt / beta-catenin signaling pathway and is a putative tumor suppressor in human HCCs. Defects in the gene encoding PRICKLE1 are associated with autosomal recessive progressive myoclonic epilepsy.

NCBI Gene ID

144165

Swiss Prot

Q96MT3

Accession Number

NP_694571

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cancer, Cell Cycle

Purification

PRICKLE1 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1310 - Human Bladder Tissue Lysate

Concentration

1 mg/mL

Buffer

PRICKLE1 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

PRICKLE1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Specificity

Multiple isoforms of PRICKLE1 are known to exist. PRICKLE1 antibody is predicted to not cross-react with other PRICKLE protein family members.

Symbol

PRICKLE1

NCBI Official Name

Prickle homolog 1

NCBI Organism

Homo sapiens

Background Reference 01

Katoh M and Katoh M. Identification and characterization of human PRICKLE1 and PRICKLE2 genes as well as mouse Prickle1 and Prickle2 genes homologous to Drosophila tissue polarity gene prickle. Int. J. Mol. Med. 2003; 11:249-56

Background Reference 02

Shimojo M and Hersh LB. REST/NRSF-interacting LIM domain protein, a putative nuclear translocation receptor. Mol. Cell. Biol. 2003; 23:9025-31

Background Reference 03

Chan DW, Chan CY, Yam JW, et al. Prickle1 negatively regulates Wnt/β -catenin pathway by promoting Disheveled ubiquitination/degradation in liver cancer. Gastroenterology 2006; 131:1218-27.