NUMB Antibody

Product Specifications

Background

NUMB Antibody: NUMB, the mammalian homolog to the Drosophila asymmetric cell fate determinant NUMB, is thought to share several features molecular mechanisms in mammalian cells, generating asymmetric cell divisions during neurogenesis in vertebrate development as well as in hematopoietic stem cells. NUMB has been shown to inhibit Notch signaling, and is itself regulated by ubiquitinylation by MDM2. NUMB has also been shown to help activate the tumor suppressor p53, suggesting that loss of NUMB in cancerous cells would not only activate the potential oncogene Notch, but diminish the tumor suppressing effect of p53.

NCBI Gene ID

8650

Swiss Prot

P49757

Accession Number

NP_1005743

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Stem Cell

Purification

NUMB Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1402 - Mouse Lung Tissue Lysate

Concentration

1 mg/mL

Buffer

NUMB Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

NUMB antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Specificity

At least four isoforms of NUMB are known to exist; this antibody will detect all of them.

Symbol

NUMB

Positive Control 2

Cat. No. 10-101 - Human Lung Tissue Slide

NCBI Official Name

Numb homolog (Drosophila)

NCBI Organism

Homo sapiens

Background Reference 01

Zhong W, Feder JN, Jiang MM, et al. Asymmetric localization of a mammalian numb homolog during mouse cortical neurogenesis. Neuron 1996; 17:43-53.

Background Reference 02

Wu M, Kwon HY, Rattis F, et al. Imaging hematopoietic precursor division in real time. Cell. Stem Cell 2007; 1:541-54.

Background Reference 03

Conboy IM and Rando TA. The regulation of Notch signaling controls satellite cell activation, cell fate determination in postnatal myogenesis. Dev. Cell 2002; 3:397-409

Background Reference 04

Yogosawa S, Miyauchi Y, Honda R, et al. Mammalian Numb is a target protein of Mdm2, ubiquitin ligase. Biochem. Biophys. Res. Commun. 2003; 302:869-72.