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SPT2 Antibody

Product Specifications

Background

SPT2 Antibody: Serine palmitoyltransferase (SPT), which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5'-phosphate as a cofactor. SPT2 is the long chain base subunit 2 of mammalian serine palmitoyltransferase. SPT2 is catalytically active but needs its related protein SPT1 for its stabilization and anchoring the holoenzyme to the cytosolic face of the endoplasmic reticulum. As in the case with SPT1, mutations in the SPT2 gene can cause hereditary sensory and autonomic neuropathy type I (HSAN-I), resulting from a loss of SPT activity as well as the accumulation of the atypical and neurotoxic sphingoid metabolite 1-deoxy-sphinganine.

NCBI Gene ID

9517

Swiss Prot

O15270

Accession Number

NP_004854

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

6305

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Validation

Independent Antibody Validation in Cell lines (Figure 1) shows similar SPT2 expression profile in human cell lines detected by two independent anti-SPT2 antibodies that recognize different epitopes, 6305 against C-terminus domain and 64-238 against central domain. SPT2 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines6305 64-238

Field of Research

Homeostasis

Purification

SPT2 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1282 - 3T3 (NIH) Cell Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Rat (100%) .

Buffer

SPT2 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

SPT2 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=6305

Fragment

IgG

Specificity

SPT2 antibody is predicted to not cross-react with SPT1.

Isoform

Human SPT2 has one isoform (562aa, 63kD) . Mouse SPT2 has one isoform (560aa, 63kD) and Rat SPT2 also has one isoform (560aa, 63kD) . 6305 can detect human, mouse and rat.

Symbol

SPTLC2

Positive Control 2

Cat. No. 17-201 - 3T3/BALB Cell Slide

Positive Control 3

Cat. No. 1204 - K562 Cell Lysate

Positive Control 4

Cat. No. 1219 - MCF7 Cell Lysate

Positive Control 5

Cat. No. 1220 - SK-N-SH Cell Lysate

NCBI Official Name

Serine palmitoyltransferase, long chain base subunit 2

NCBI Organism

Homo sapiens

Background Reference 01

Batheja et al. J. Histochem. & Cytochem. 2003; 51:687-96.

Background Reference 02

Nagiec et al. Gene 1996; 177:237-41

Background Reference 03

Yasuda et al. J. Biol. Chem. 2003; 278:4176-83.

Background Reference 04

Rotthier et al. Am. J. Hum. Genet. 2010; 87:513-22.

Other Product Names

SPT2 Antibody: LCB2, SPT2, HSN1C, LCB2A, NSAN1C, hLCB2a, KIAA0526, LCB2, Serine palmitoyltransferase 2, Long chain base biosynthesis protein 2, LCB 2

Tested Applications

ELISA, WB, ICC, IF

Protein ID

4758668

Physical Properties

Liquid

Available Sizes

Curated Selection

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