SIK1 Antibody

Product Specifications

Background

SIK1 Antibody: Salt-inducible kinase 1 (SIK1), also known as SNF1LK or MSK, plays a role in histone modification and G2/M cell cycle regulation. It is a 783 amino acid protein that contains one UBA domain and belongs to the Ser/Thr protein kinase family (AMPK subfamily) . Localized to both the nucleus and the cytoplasm, SIK1 is a class II HDAC kinase that uses magnesium as a cofactor to catalyze the ATP-dependent phosphorylation of target proteins and is thought to be important for the early stages of skeletal muscle growth and myocardial cell differentiation.

NCBI Gene ID

150094

Swiss Prot

P57059

Accession Number

NP_775490

Host

Rabbit

Reactivity

Human

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Signal Transduction

Purification

SIK1 Antibody is affinity chromatography purified via peptide column.

Positive Control

Cat. No. 1308 - Human Small Intestine Tissue Lysate

Concentration

1 mg/mL

Homology

Predicted species reactivity based on immunogen sequence: Rat: (73%), Mouse: (73%)

Buffer

SIK1 Antibody is supplied in PBS containing 0.02% sodium azide.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

SIK1 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Fragment

IgG

Specificity

Two isoforms of SIK1 are known to exist; this antibody will detect both isoforms. SIK1 antibody is predicted to not cross-react with other SIK protein family members.

Symbol

SIK1

Positive Control 2

Cat. No. 11-801 - Human Small Intestine Tissue Slide

NCBI Official Name

Salt-inducible kinase 1

NCBI Organism

Homo sapiens

Background Reference 01

Stephenson A, Huang GY, Nguyen NT, et al. SNF1LK encodes a protein kinase that may function in cell cycle regulation. Genomics 2004; 83:1105-15

Background Reference 02

Ruiz JC, Conlon FL, and Robertson EJ. Identification of novel protein kinases expressed in the myocardium of the developing mouse heart. Mech. Dev. 1994; 48:153-64

Background Reference 03

Lizcano JM, Goransson O, Toth R, et al. LKB1 is a master kinase that activates 13 kinases of the AMPK subfamily, including MARK/PAR-1. EMBO J. 2004; 23:833-43.

Background Reference 04

Sjö strö m M, Senstrom K, Eneling K, et al. SIK1 is part of a cell sodium-sensing network that regulates active sodium transport through a calcium-dependent process. Proc. Natl. Acad. Sci. USA 2007; 104:16922-7.